Biochemistry Laboratory: Modern Theory and Techniques, 2nd edition

Published by Pearson (November 21, 2011) © 2012

  • Rodney Boyer
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The biochemistry laboratory course is an essential component in training students for careers in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Increasingly, many biochemistry lab instructors opt to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
  • Chapter 1: Introduction to the Biochemistry/Molecular Biology Laboratory
  • Chapter 2: Using the Computer and Internet for Research in Biochemistry
  • Chapter 3: General Laboratory Procedures
  • Chapter 4: Centrifugation Techniques in Biochemistry/Molecular Biology
  • Chapter 5: Purification and Analysis of Biomolecules by Chromatography
  • Chapter 6: Characterization of Proteins and Nucleic Acids by Electrophoresis
  • Chapter 7: Spectroscopic Analysis of Biomolecules
  • Chapter 8: Biomolecular Interactions: Ligand Binding and Enzyme Reacations
  • Chapter 9: Molecular Biology I: Structures and Analysis of Nucleic Acids
  • Chapter 10: Molecular Biology II: Recombinant DNA, Molecular Cloning and Enzymology
  • Chapter 11: Protein Production, Purification, and Characterization
  • Appendices
  • Index

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