In this video, we're going to introduce pili. And so the surface of bacterial cells can have relatively long filamentous protein structures that are called pili. And so pili is actually the plural form of the word. The singular form is pilus. And so these pili are protein filaments that extend from the surface of the cell. And these extensions can have varied functions. And so if we take a look at this image down below, notice that we're showing you a bacterial cell right here and extending off is this long filamentous protein structure that we call a pilus. And the pilus can have varied functions depending on the cell type. And so we'll get to talk about some of the main types of functions of pili in our next lesson video. So I'll see you all there.
- 1. Introduction to Microbiology3h 21m
- Introduction to Microbiology16m
- Introduction to Taxonomy26m
- Scientific Naming of Organisms9m
- Members of the Bacterial World10m
- Introduction to Bacteria9m
- Introduction to Archaea10m
- Introduction to Eukarya20m
- Acellular Infectious Agents: Viruses, Viroids & Prions19m
- Importance of Microorganisms20m
- Scientific Method27m
- Experimental Design30m
- 2. Disproving Spontaneous Generation1h 18m
- 3. Chemical Principles of Microbiology3h 38m
- 4. Water1h 28m
- 5. Molecules of Microbiology2h 23m
- 6. Cell Membrane & Transport3h 28m
- Cell Envelope & Biological Membranes12m
- Bacterial & Eukaryotic Cell Membranes8m
- Archaeal Cell Membranes18m
- Types of Membrane Proteins8m
- Concentration Gradients and Diffusion9m
- Introduction to Membrane Transport14m
- Passive vs. Active Transport13m
- Osmosis33m
- Simple and Facilitated Diffusion17m
- Active Transport30m
- ABC Transporters11m
- Group Translocation7m
- Types of Small Molecule Transport Review9m
- Endocytosis and Exocytosis15m
- 7. Prokaryotic Cell Structures & Functions5h 52m
- Prokaryotic & Eukaryotic Cells26m
- Binary Fission11m
- Generation Times16m
- Bacterial Cell Morphology & Arrangements35m
- Overview of Prokaryotic Cell Structure10m
- Introduction to Bacterial Cell Walls26m
- Gram-Positive Cell Walls11m
- Gram-Negative Cell Walls20m
- Gram-Positive vs. Gram-Negative Cell Walls11m
- The Glycocalyx: Capsules & Slime Layers12m
- Introduction to Biofilms6m
- Pili18m
- Fimbriae & Hami7m
- Introduction to Prokaryotic Flagella12m
- Prokaryotic Flagellar Structure18m
- Prokaryotic Flagellar Movement11m
- Proton Motive Force Drives Flagellar Motility5m
- Chemotaxis14m
- Review of Prokaryotic Surface Structures8m
- Prokaryotic Ribosomes16m
- Introduction to Bacterial Plasmids13m
- Cell Inclusions9m
- Endospores16m
- Sporulation5m
- Germination5m
- 8. Eukaryotic Cell Structures & Functions2h 18m
- 9. Microscopes2h 46m
- Introduction to Microscopes8m
- Magnification, Resolution, & Contrast10m
- Introduction to Light Microscopy5m
- Light Microscopy: Bright-Field Microscopes23m
- Light Microscopes that Increase Contrast16m
- Light Microscopes that Detect Fluorescence16m
- Electron Microscopes14m
- Reviewing the Different Types of Microscopes10m
- Introduction to Staining5m
- Simple Staining14m
- Differential Staining6m
- Other Types of Staining11m
- Reviewing the Types of Staining8m
- Gram Stain13m
- 10. Dynamics of Microbial Growth4h 36m
- Biofilms16m
- Growing a Pure Culture5m
- Microbial Growth Curves in a Closed System21m
- Temperature Requirements for Microbial Growth18m
- Oxygen Requirements for Microbial Growth22m
- pH Requirements for Microbial Growth8m
- Osmolarity Factors for Microbial Growth14m
- Reviewing the Environmental Factors of Microbial Growth12m
- Nutritional Factors of Microbial Growth30m
- Growth Factors4m
- Introduction to Cultivating Microbial Growth5m
- Types of Solid Culture Media4m
- Plating Methods16m
- Measuring Growth by Direct Cell Counts9m
- Measuring Growth by Plate Counts14m
- Measuring Growth by Membrane Filtration6m
- Measuring Growth by Biomass15m
- Introduction to the Types of Culture Media5m
- Chemically Defined Media3m
- Complex Media4m
- Selective Media5m
- Differential Media9m
- Reducing Media4m
- Enrichment Media7m
- Reviewing the Types of Culture Media8m
- 11. Controlling Microbial Growth4h 10m
- Introduction to Controlling Microbial Growth29m
- Selecting a Method to Control Microbial Growth44m
- Physical Methods to Control Microbial Growth49m
- Review of Physical Methods to Control Microbial Growth7m
- Chemical Methods to Control Microbial Growth16m
- Chemicals Used to Control Microbial Growth6m
- Liquid Chemicals: Alcohols, Aldehydes, & Biguanides15m
- Liquid Chemicals: Halogens12m
- Liquid Chemicals: Surface-Active Agents17m
- Other Types of Liquid Chemicals14m
- Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde13m
- Review of Chemicals Used to Control Microbial Growth11m
- Chemical Preservation of Perishable Products10m
- 12. Microbial Metabolism5h 16m
- Introduction to Energy15m
- Laws of Thermodynamics15m
- Chemical Reactions9m
- ATP20m
- Enzymes14m
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- Enzyme Binding Factors9m
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- Introduction to Metabolism8m
- Negative & Positive Feedback7m
- Redox Reactions22m
- Introduction to Aerobic Cellular Respiration25m
- Types of Phosphorylation12m
- Glycolysis19m
- Entner-Doudoroff Pathway11m
- Pentose-Phosphate Pathway10m
- Pyruvate Oxidation8m
- Krebs Cycle16m
- Electron Transport Chain19m
- Chemiosmosis7m
- Review of Aerobic Cellular Respiration19m
- Fermentation & Anaerobic Respiration23m
- 13. Photosynthesis2h 31m
- 14. DNA Replication2h 25m
- 15. Central Dogma & Gene Regulation7h 14m
- Central Dogma7m
- Introduction to Transcription20m
- Steps of Transcription22m
- Transcription Termination in Prokaryotes7m
- Eukaryotic RNA Processing and Splicing20m
- Introduction to Types of RNA9m
- Genetic Code25m
- Introduction to Translation30m
- Steps of Translation23m
- Review of Transcription vs. Translation12m
- Prokaryotic Gene Expression21m
- Review of Prokaryotic vs. Eukaryotic Gene Expression13m
- Introduction to Regulation of Gene Expression13m
- Prokaryotic Gene Regulation via Operons27m
- The Lac Operon21m
- Glucose's Impact on Lac Operon25m
- The Trp Operon20m
- Review of the Lac Operon & Trp Operon11m
- Introduction to Eukaryotic Gene Regulation9m
- Eukaryotic Chromatin Modifications16m
- Eukaryotic Transcriptional Control22m
- Eukaryotic Post-Transcriptional Regulation28m
- Post-Translational Modification6m
- Eukaryotic Post-Translational Regulation13m
- 16. Microbial Genetics4h 44m
- Introduction to Microbial Genetics11m
- Introduction to Mutations20m
- Methods of Inducing Mutations15m
- Prototrophs vs. Auxotrophs13m
- Mutant Detection25m
- The Ames Test14m
- Introduction to DNA Repair5m
- DNA Repair Mechanisms37m
- Horizontal Gene Transfer18m
- Bacterial Transformation11m
- Transduction32m
- Introduction to Conjugation6m
- Conjugation: F Plasmids18m
- Conjugation: Hfr & F' Cells19m
- Genome Variability21m
- CRISPR CAS11m
- 17. Biotechnology3h 0m
- 18. Viruses, Viroids, & Prions4h 56m
- Introduction to Viruses20m
- Introduction to Bacteriophage Infections14m
- Bacteriophage: Lytic Phage Infections12m
- Bacteriophage: Lysogenic Phage Infections17m
- Bacteriophage: Filamentous Phage Infections8m
- Plaque Assays9m
- Introduction to Animal Virus Infections10m
- Animal Viruses: 1. Attachment to the Host Cell7m
- Animal Viruses: 2. Entry & Uncoating in the Host Cell19m
- Animal Viruses: 3. Synthesis & Replication22m
- Animal Viruses: DNA Virus Synthesis & Replication14m
- Animal Viruses: RNA Virus Synthesis & Replication22m
- Animal Viruses: Antigenic Drift vs. Antigenic Shift9m
- Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication9m
- Animal Viruses: 4. Assembly Inside Host Cell8m
- Animal Viruses: 5. Release from Host Cell15m
- Acute vs. Persistent Viral Infections25m
- COVID-19 (SARS-CoV-2)14m
- Plant Viruses12m
- Viroids6m
- Prions13m
- 19. Innate Immunity7h 15m
- Introduction to Immunity8m
- Introduction to Innate Immunity17m
- Introduction to First-Line Defenses5m
- Physical Barriers in First-Line Defenses: Skin13m
- Physical Barriers in First-Line Defenses: Mucous Membrane9m
- First-Line Defenses: Chemical Barriers24m
- First-Line Defenses: Normal Microflora5m
- Introduction to Cells of the Immune System15m
- Cells of the Immune System: Granulocytes29m
- Cells of the Immune System: Agranulocytes25m
- Introduction to Cell Communication5m
- Cell Communication: Surface Receptors & Adhesion Molecules16m
- Cell Communication: Cytokines27m
- Pattern Recognition Receptors (PRRs)45m
- Introduction to the Complement System24m
- Activation Pathways of the Complement System23m
- Effects of the Complement System23m
- Review of the Complement System12m
- Phagoctytosis21m
- Introduction to Inflammation18m
- Steps of the Inflammatory Response26m
- Fever8m
- Interferon Response25m
- 20. Adaptive Immunity7h 14m
- Introduction to Adaptive Immunity32m
- Antigens12m
- Introduction to T Lymphocytes38m
- Major Histocompatibility Complex Molecules20m
- Activation of T Lymphocytes21m
- Functions of T Lymphocytes25m
- Review of Cytotoxic vs Helper T Cells13m
- Introduction to B Lymphocytes27m
- Antibodies14m
- Classes of Antibodies35m
- Outcomes of Antibody Binding to Antigen15m
- T Dependent & T Independent Antigens21m
- Clonal Selection20m
- Antibody Class Switching17m
- Affinity Maturation14m
- Primary and Secondary Response of Adaptive Immunity21m
- Immune Tolerance28m
- Regulatory T Cells10m
- Natural Killer Cells16m
- Review of Adaptive Immunity25m
- 21. Principles of Disease6h 57m
- Symbiotic Relationships12m
- The Human Microbiome46m
- Characteristics of Infectious Disease47m
- Stages of Infectious Disease Progression26m
- Koch's Postulates26m
- Molecular Koch's Postulates11m
- Bacterial Pathogenesis36m
- Introduction to Pathogenic Toxins6m
- Exotoxins Cause Damage to the Host40m
- Endotoxin Causes Damage to the Host13m
- Exotoxins vs. Endotoxin Review13m
- Immune Response Damage to the Host15m
- Introduction to Avoiding Host Defense Mechanisms8m
- 1) Hide Within Host Cells5m
- 2) Avoiding Phagocytosis31m
- 3) Surviving Inside Phagocytic Cells10m
- 4) Avoiding Complement System9m
- 5) Avoiding Antibodies25m
- Viruses Evade the Immune Response27m
Pili: Study with Video Lessons, Practice Problems & Examples
Pili, or pili, are filamentous protein structures on bacterial cells with key functions in motility and DNA transfer. They facilitate twitching motility</>, where the pilus extends and retracts to move the cell, and gliding motility</>, which allows smooth movement. The sex pilus</>, or conjugation pilus, enables direct DNA transfer between cells, enhancing traits like antibiotic resistance. Understanding these mechanisms is crucial for grasping bacterial behavior and genetic exchange.
Pili
Video transcript
Functions of Pili
Video transcript
In this video, we're going to talk about some of the functions of pili. Pili typically number in only 1 to 2 per cell. Although pili can have many different functions, generally pili have 2 primary functions that we have numbered down below, 12.
The first primary function of pili is cell motility. Motility is really just referring to the ability of an organism to move through its environment. You can see that in this image, the bacterial cell here is using its pilus to move from the starting point to the finish line over here. Pili can be used for cell motility, for a cell to propel itself and move itself through its environment.
The second primary function of pili is for DNA transfer. The pilus can be used as somewhat of a bridge to connect one cell to a neighboring cell. This pilus can be used as a bridge or a tunnel to transfer DNA from one cell, and transfer that DNA to a neighboring cell. You can see here that the DNA is almost like a gift, and bacterial cells are able to exchange DNA with neighboring cells by using their pili.
This is something that we'll be able to talk more about as we move forward in our course. But for now, this here concludes our brief introduction to the functions of pili. I'll see you all in our next video.
Which of these are true about pili?
Cell Motility by Pili
Video transcript
In this video, we're going to talk about how pili can be used for cell motility or cell movement. Pili are actually involved in two types of cell motility that we have numbered down below: 12. The first type of cell motility in which pili are involved is twitching motility. Twitching motility can be described as the pili itself extending from the cell, attaching to a surface, and then retracting in order to drag the cell forward. During this period, the cell is actually moving towards its destination in a twitching-like or a jerking-like motion. This is where it gets its name, twitching motility. Notice that with this, the bacteria will be extending its pilus, attaching its pilus to an area that's further along the destination, and then retracting its pilus to pull the bacterial cell forwards in the direction of its movement. It is going to be a twitching or a jerking type of movement that is not going to be continuous and will appear very twitch-like.
On the other hand, the second type of motility that pili are involved in is gliding motility. In gliding motility, there is going to be a smooth movement that does not have a twitch-like or a jerk-like effect. The movement of the cell along the axis will be smooth. There are motor proteins involved with this process that will attach to the surface surrounding the cell. However, the exact mechanism of this is unknown. Looking at our image down below at the gliding motility, you will notice that there are motor proteins involved, and the pili is also going to be involved in some way, but ultimately the cell movement is going to be very smooth and will not be twitch-like. You can see we have the motor proteins labeled here. The significant difference is that both twitching motility and gliding motility somehow involve a pilus. Twitching motility is going to be in a twitch-like or jerk-like fashion, whereas gliding motility is going to be in a smooth-like fashion.
This concludes our brief introduction to cell motility by pili, and we'll be able to get some practice as we move forward. I'll see you all in our next video.
________ is the mechanism where a bacterial cell uses its pili to crawl across a surface towards a destination:
Scientists believe some bacteria are able to 'glide' through their environment by…
Which cellular structures are involved in conjugation?
Sex Pilus
Video transcript
In this video, we're going to introduce the sex pilus. The sex pilus is also referred to as the conjugation pilus. It connects two cells directly for a special type of DNA transfer known as conjugation. Conjugation can be defined as the process by which DNA is transferred from one bacterial cell to another by using direct contact through the sex pilus or the conjugation pilus. This transferred DNA, occurring via conjugation, can add new functions to a cell, such as resistance to antibiotics.
Looking at the image below, we can better understand how the sex pilus can bring two cells together to directly transfer genetic material. In this part of the image, we see two bacterial cells; bacterial cell A and bacterial cell B. Bacterial cell A has a yellow circle representing a DNA plasmid, possibly conferring antibiotic resistance. However, cell B does not have this yellow circle, indicating sensitivity to antibiotics.
The green structure seen here is the sex pilus itself, which brings the two cells into closer proximity. This action allows the DNA plasmid to be copied and transferred to cell B. Both cells A and B eventually possess the plasmid, highlighting its transfer through the action of the sex pilus. Now, cell B can also transfer this DNA to other cells. This is accomplished through the action of their own sex pili, demonstrating the recurring potential of this process. This concludes our lesson here, and I'll see you all in our next video.
Which of the following is NOT a function of pili?
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More setsHere’s what students ask on this topic:
What are pili and what are their main functions?
Pili are filamentous protein structures that extend from the surface of bacterial cells. They serve several key functions, primarily in motility and DNA transfer. Pili can facilitate two types of motility: twitching motility, where the pilus extends, attaches to a surface, and retracts to drag the cell forward in a jerking motion, and gliding motility, which allows smooth movement along a surface. Additionally, pili play a crucial role in DNA transfer through a process called conjugation. The sex pilus, or conjugation pilus, connects two bacterial cells, enabling the transfer of genetic material, such as antibiotic resistance genes, from one cell to another.
How do pili contribute to bacterial motility?
Pili contribute to bacterial motility through two main mechanisms: twitching motility and gliding motility. In twitching motility, the pilus extends from the bacterial cell, attaches to a surface, and then retracts, pulling the cell forward in a jerking or twitching motion. This type of movement is discontinuous and appears as a series of short, rapid movements. In contrast, gliding motility involves a smooth, continuous movement along a surface. Although the exact mechanism of gliding motility is not fully understood, it is known to involve motor proteins and pili, allowing the cell to move in a more fluid manner.
What is the role of the sex pilus in bacterial conjugation?
The sex pilus, also known as the conjugation pilus, plays a crucial role in bacterial conjugation, a process of direct DNA transfer between bacterial cells. The sex pilus connects two cells, bringing them into close proximity. This connection allows the transfer of genetic material, such as plasmids, from one cell to another. For example, a plasmid carrying antibiotic resistance genes can be transferred from a donor cell to a recipient cell, potentially conferring new traits to the recipient. This process is essential for genetic diversity and the spread of advantageous traits among bacterial populations.
What are the differences between twitching motility and gliding motility in bacteria?
Twitching motility and gliding motility are two distinct types of bacterial movement facilitated by pili. Twitching motility involves the extension of the pilus, attachment to a surface, and retraction, which pulls the cell forward in a jerking or twitching motion. This movement is discontinuous and appears as a series of short, rapid movements. In contrast, gliding motility is characterized by smooth, continuous movement along a surface. Although the exact mechanism of gliding motility is not fully understood, it involves motor proteins and pili, allowing the cell to move in a more fluid manner without the jerking motion seen in twitching motility.
How does the transfer of DNA via the sex pilus affect bacterial cells?
The transfer of DNA via the sex pilus, or conjugation pilus, can significantly impact bacterial cells by introducing new genetic material. This process, known as conjugation, allows a donor cell to transfer plasmids carrying specific genes, such as those for antibiotic resistance, to a recipient cell. The recipient cell can then express these new genes, potentially gaining new functions or traits. For example, a recipient cell that acquires a plasmid with antibiotic resistance genes can become resistant to certain antibiotics, enhancing its survival in environments with antibiotic presence. This genetic exchange contributes to bacterial diversity and adaptability.