In this video, we're going to introduce our map of the lesson on DNA-based technologies, which is down below right here. This image really is and can be used like a map to help guide you as we move forward through our lesson on DNA-based technologies. Notice at the very top we have our DNA-based technologies, and we've got these branches. The way this map is going to work is we are always going to be following the leftmost branches first and explore them to their end. We'll start off over here exploring all of this, then once we finish exploring all of that we'll zoom out and explore the next leftmost branch. Then we'll continue on in this pattern as you see like this. And last but not least, we'll cover this last region over here.
First, as we cover DNA-based technologies, we are going to talk about DNA cloning, which you can see over here. We've got DNA cloning lesson broken up into two parts. The first part is creating the recombinant DNA molecule, which involves using restriction enzymes to cut the DNA, which pretty much act like little tiny molecular scissors. We'll talk about that more as we move forward. It also involves the ligation enzymes that are going to help paste the DNA. Here we have some DNA, and we are going to cut and paste to create a recombinant DNA. The second step is going to be transforming that recombinant DNA into bacteria. Again, we're going to talk all about this in more detail as we move forward in our course.
After discussing DNA cloning, we'll talk about the next branch here, which is going to be polymerase chain reaction, abbreviated as PCR, and we'll discuss the steps of PCR, which includes denaturation, annealing, and extension.
Following that, we'll move on to talk about DNA-based technologies that are used for the separation of DNA samples. This includes gel electrophoresis, southern blotting, and DNA fingerprinting. After we discuss the separation of DNA samples, we'll move on to the final section here of our map of the lesson, and that is going to be about DNA sequencing.
We're going to talk specifically about dideoxy sequencing, involving chain termination PCR and also determining the DNA sequence. This is really the map of our entire lesson on DNA-based technologies. Again, you can use this map as you move forward as a guide to help you make predictions about what we're going to talk about next, and to help make sure that you know where we are within our lesson. This here concludes our introduction to our map of DNA-based technology lesson, and I'll see you all in our next video.