In this video, we're going to talk more about the details of step number 1 of DNA cloning, which is creating the recombinant DNA. Creating a recombinant DNA molecule is actually a two-step process within itself that requires step 1a and step 1b.
Now, step 1a involves using restriction enzymes to cut the DNA. These restriction enzymes act like tiny molecular scissors because they can cut the DNA molecule. In the image, we use scissors to represent the restriction enzymes, showing how they cut the DNA. After the DNA has been cut, the second part, step 1b, involves using ligase enzymes. Ligase enzymes act like glue, covalently linking the cut DNA molecules. Here, we depict this with a little bottle of glue symbolizing the ligase enzymes, which paste together the cut bacterial plasmid and the gene of interest.
At this point, we have the final result, which is the recombinant DNA, concluding step number 1, the creation of recombinant DNA. This concludes this video, and we will move on to step number 2 in our next video.