The melting temperature of DNA is the temperature at which half of the DNA strands are in a random coil or single-stranded configuration. Determine whether the following statement is true or false, and state your reasoning.
Longer strands of DNA have a lower melting temperature than shorter ones.

• A. The statement is true. The longer the DNA strand, the less effective nucleotides stacking is expected, lowering DNA stability and melting temperature.
• B. The statement is false. The longer the DNA strand, the more hydrogen bonding is expected, increasing DNA stability and melting temperature.

Answer: (B)