GOB Chemistry
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What impact would you predict on the rate of an enzymatic process for an enzyme that operates optimally at 25°C (room temperature), if the temperature is increased from 25°C to 55°C?
What general effect(s) might adding a polar organic solvent like ethanol have on an enzymatic process occurring at optimal human body temperature (around 37°C)?
What is the implication when we say that the enzyme is fully occupied by a substrate? How does adding more enzyme and substrate influence the rate of the chemical reaction?
What would be the likely impact on the rate of a biochemical reaction catalyzed by an enzyme that functions optimally at room temperature (about 25°C) if a reducing agent like dithiothreitol (DTT) is introduced?
Lipase, an enzyme found in the pancreas, exhibits high catalytic activity at pH 8, while pepsin, an enzyme found in the stomach, exhibits no catalytic activity at this pH. Why?
How would the rate of an enzyme-catalyzed reaction be affected if the amount of the enzyme was increased two-fold? Explain why.
In a biochemistry lab, students conducted an experiment to compare the enzyme activity in spinach and lettuce leaves. They first observed the color change in both leaves when exposed to air due to phenolase activity. Then, they performed another experiment where they added hydrogen peroxide (H2O2) to fresh spinach and lettuce samples and recorded the time until bubbles appeared. Which sample has more catalase? Explain your answer.
Kiwi's actinidin enzyme hydrolyzes peptide bonds in proteins. In the process of making a soy milk-based pudding, the recipe advises against using fresh kiwi. However, it mentions that kiwi juice concentrate, which undergoes pasteurization, is acceptable. What is the reason for this recommendation?
Papaya has papain that can hydrolyze peptide bonds in the proteins. In the process of marinating a tough meat, why is fresh papaya used?
True or false: Enzymes perform well under extreme conditions.
Amylase, an enzyme that catalyzes the breakdown of starches into sugars, operates optimally in the mouth at a pH between 6.7 and 7.0. How would the rate of an amylase-catalyzed reaction be affected by changing the pH to 4.0? Select the best answer.
The graph below illustrates the relationship between enzyme activity and pH for catalase, peptidase, and cellulase. Based on the graph, which of the following optimal pH is correct?
Using the provided graph, identify which of the following conditions is closest to the optimum reaction rate. Select the best answer.
Chymotrypsin, an enzyme that facilitates peptide bond hydrolysis, functions optimally in the small intestine at a pH of 7.5 to 8.5. How would the enzyme's activity be impacted under the following condition? (Select the best answer.)
Condition: Conducting the reaction at 90 °C.
Amylase is an enzyme that catalyzes the hydrolysis of starch into sugars in the human mouth and pancreas. Its optimal activity is at a pH of 6.7 to 7.0. What would be the effect on the rate of an amylase-catalyzed reaction if the pH was adjusted to 4.5?