18. Molecular Genetic Tools
Genetic Cloning
18. Molecular Genetic Tools
Genetic Cloning - Video Tutorials & Practice Problems
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Genetic Cloning
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Problem
ProblemThe purpose of polymerase chain reaction is to do what?
A
Create RNA templates
B
Create fluorescent probes
C
Amplify a short DNA sequence
D
Isolate proteins
3
Problem
ProblemWhich of the following lists the steps of genetic cloning in the proper order?
A
PCR → Restriction Enzyme Cutting → Ligate into a Vector → Placed into Organism
B
Restriction Enzyme Cutting → PCR → Ligate into a Vector → Placed into Organism
C
Ligate into a Vector → PCR → Restriction Enzyme Cutting → Placed into Organism
D
PCR → Ligate into a Vector → Restriction Enzyme Cutting → Placed into Organism
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PRACTICE PROBLEMS AND ACTIVITIES (81)
- In this chapter we focused on how specific DNA sequences can be copied, identified, characterized, and sequenc...
- In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biot...
- In this chapter we focused on how specific DNA sequences can be copied, identified, characterized, and sequenc...
- In this chapter we focused on how specific DNA sequences can be copied, identified, characterized, and sequenc...
- In this chapter we focused on how specific DNA sequences can be copied, identified, characterized, and sequenc...
- What purpose do the bla and lacZ genes serve in the plasmid vector pUC18?
- The human genome is 3×10⁹ bp in length. How many fragments would be predicted to result from the complete dig...
- The human genome is 3×10⁹ bp in length. How would your initial answer change if you knew that the average GC ...
- Write a short essay or sketch a diagram that provides an overview of how recombinant DNA techniques help genet...
- Write a short essay that summarizes the impacts that genomic applications are having on society and discuss wh...
- What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA...
- Why are most recombinant human proteins produced in animal or plant hosts instead of bacterial host cells?
- Ligase catalyzes a reaction between the 5′ phosphate and the 3′ hydroxyl groups at the ends of DNA molecules. ...
- One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica...
- One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica...
- The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcrip...
- Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other c...
- The human genome is 3×10⁹ bp. You wish to design a primer to amplify a specific gene in the genome. In general...
- Using DNA sequencing on a cloned DNA segment, you recover the nucleotide sequence shown below. Does this segme...
- Using animal models of human diseases can lead to insights into the cellular and genetic bases of the diseases...
- Restriction sites are palindromic; that is, they read the same in the 5' to 3' direction on each strand of DNA...
- Using animal models of human diseases can lead to insights into the cellular and genetic bases of the diseases...
- List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs p...
- Compare methods for constructing homologous recombinant transgenic mice and yeast.
- What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would yo...
- Chimeric gene-fusion products can be used for medical or industrial purposes. One idea is to produce biologica...
- Outline the roles played by restriction enzymes and vectors in cloning DNA.
- In 1975, the Asilomar Conference on Recombinant DNA was organized by Paul Berg, a pioneer of recombinant DNA t...
- Why are diseases of the blood simpler targets for treatment by gene therapy than are many other genetic diseas...
- Injection of double-stranded RNA can lead to gene silencing by degradation of RNA molecules complementary to e...
- What are some of the impacts of biotechnology on crop plants in the United States?
- In the context of recombinant DNA technology, of what use is a probe?
- Compare and contrast methods for making transgenic plants and transgenic Drosophila.
- Summarize the arguments for and against patenting genetically modified organisms.
- If you performed a PCR experiment starting with only one copy of double-stranded DNA, approximately how many D...
- It is often desirable to insert cDNAs into a cloning vector in such a way that all the cDNA clones will have t...
- A major advance in the 1980s was the development of technology to synthesize short oligonucleotides. This work...
- The bacteriophage lambda genome can exist in either a linear form (see Figures 15.1 and 15.8) or a circular fo...
- You have recovered a cloned DNA segment from a vector and determine that the insert is 1300 bp in length. To c...
- The restriction enzymes XhoI and SalI cut their specific sequences as shown below:Xhol 5'-C TC...
- The bacteriophage ϕX174 has a single-stranded DNA genome of 5386 bases. During DNA replication, double-strande...
- The Age of Genetics was created by remarkable advances in the use of biotechnology to manipulate plant and ani...
- To further analyze the CRABS CLAW gene (see Problems 19 and 20), you create a map of the genomic clone. The 11...
- To estimate the number of cleavage sites in a particular piece of DNA with a known size, you can apply the for...
- In Genetic Analysis 14.1 we designed a screen to identify conditional mutants of S. cerevisiae in which the se...
- In Genetic Analysis 14.1 we designed a screen to identify conditional mutants of S. cerevisiae in which the se...
- You have isolated a genomic clone with an EcoRI fragment of 11 kb that encompasses the CRABS CLAW gene (see Pr...
- You have isolated a genomic clone with an EcoRI fragment of 11 kb that encompasses the CRABS CLAW gene (see Pr...
- In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92-26 °C, ...
- You have identified a 0.80-kb cDNA clone that contains the entire coding sequence of the Arabidopsis gene CRAB...
- We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is subjected to...
- Traditional Sanger sequencing has largely been replaced in recent years by next-generation and third-generatio...
- Given your knowledge of the genetic tools for studying Drosophila, outline a method by which you could clone t...
- How is fluorescent in situ hybridization (FISH) used to produce a spectral karyotype?
- You have generated three transgenic lines of maize that are resistant to the European corn borer, a significan...
- How would you clone a gene that you have identified by a mutant phenotype in Drosophila?
- Bacterial Pseudomonas species often possess plasmids encoding genes involved in the catabolism of organic comp...
- Microsatellites are currently exploited as markers for paternity testing. A sample paternity test is shown in ...
- Two complaints about some transgenic plants presently in commercial use are that (1) the Bt toxin gene is cons...
- The highlighted sequence shown below is the one originally used to produce the B chain of human insulin in E. ...
- As you will learn later in the text (Special Topics Chapter 1— CRISPR-Cas and Genome Editing), the CRISPR-Cas ...
- Vitamin E is the name for a set of chemically related tocopherols, which are lipid-soluble compounds with anti...
- The gel presented here shows the pattern of bands of fragments produced with several restriction enzymes. The ...
- The gel presented here shows the pattern of bands of fragments produced with several restriction enzymes. The ...
- The RAS gene encodes a signaling protein that hydrolyzes GTP to GDP. When bound by GDP, the RAS protein is ina...
- A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the...
- You have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temperatu...
- You have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temperatu...
- Most of the techniques described in this chapter (blotting, cloning, PCR, etc.) are dependent on hybridization...
- Most of the techniques described in this chapter (blotting, cloning, PCR, etc.) are dependent on hybridization...
- About 1% of occurrences of nonautoimmune type 1 diabetes are due to loss-of-function alleles in the insulin ge...
- In humans, congenital heart disease is a common birth defect that affects approximately 1 out of 125 live birt...
- In humans, congenital heart disease is a common birth defect that affects approximately 1 out of 125 live birt...
- In humans, congenital heart disease is a common birth defect that affects approximately 1 out of 125 live birt...
- Describe how having the Cas9 gene at a genomic locus unlinked to the guide RNA and target site locus in an eng...
- The U.S. Department of Justice has established a database that catalogs PCR amplification products from short ...
- The U.S. Department of Justice has established a database that catalogs PCR amplification products from short ...
- The U.S. Department of Justice has established a database that catalogs PCR amplification products from short ...
- The U.S. Department of Justice has established a database that catalogs PCR amplification products from short ...
- Would a gene drive system spread rapidly through a population in a species that tends to self-mate (e.g., Arab...
- There are a variety of circumstances under which rapid results using multiple markers in PCR amplifications ar...