Table of contents
- 1. Introduction to Genetics(0)
- 2. Mendel's Laws of Inheritance(0)
- 3. Extensions to Mendelian Inheritance(0)
- 4. Genetic Mapping and Linkage(0)
- 5. Genetics of Bacteria and Viruses(0)
- 6. Chromosomal Variation(0)
- 7. DNA and Chromosome Structure(0)
- 8. DNA Replication(0)
- 9. Mitosis and Meiosis(0)
- 10. Transcription(0)
- 11. Translation(0)
- 12. Gene Regulation in Prokaryotes(0)
- 13. Gene Regulation in Eukaryotes(0)
- 14. Genetic Control of Development(0)
- 15. Genomes and Genomics(0)
- 16. Transposable Elements(0)
- 17. Mutation, Repair, and Recombination(0)
- 18. Molecular Genetic Tools(0)
- 19. Cancer Genetics(0)
- 20. Quantitative Genetics(0)
- 21. Population Genetics(0)
- 22. Evolutionary Genetics(0)
18. Molecular Genetic Tools
Genetic Cloning
18. Molecular Genetic Tools
Genetic Cloning: Study with Video Lessons, Practice Problems & Examples
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57PRACTICE PROBLEM
A 1500-bp cloned DNA segment was recovered from a vector, and its restriction map must be constructed in order to characterize this cloned segment. The cloned segment was digested with two restriction enzymes and then electrophoresed on agarose gel. The band sizes observed on the gel are as follows: Enzyme A: 450 bp, 1050 bp; Enzyme B: 200 bp, 1300 bp; Enzymes A and B: 200 bp, 250 bp, 1050 bp. Using this information, create a restriction map and then calculate the distance between the cutting sites of enzymes A and B (in bp) on the cloned DNA segment.
A 1500-bp cloned DNA segment was recovered from a vector, and its restriction map must be constructed in order to characterize this cloned segment. The cloned segment was digested with two restriction enzymes and then electrophoresed on agarose gel. The band sizes observed on the gel are as follows: Enzyme A: 450 bp, 1050 bp; Enzyme B: 200 bp, 1300 bp; Enzymes A and B: 200 bp, 250 bp, 1050 bp. Using this information, create a restriction map and then calculate the distance between the cutting sites of enzymes A and B (in bp) on the cloned DNA segment.