Table of contents
- 1. Introduction to Genetics(0)
- 2. Mendel's Laws of Inheritance(0)
- 3. Extensions to Mendelian Inheritance(0)
- 4. Genetic Mapping and Linkage(0)
- 5. Genetics of Bacteria and Viruses(0)
- 6. Chromosomal Variation(0)
- 7. DNA and Chromosome Structure(0)
- 8. DNA Replication(0)
- 9. Mitosis and Meiosis(0)
- 10. Transcription(0)
- 11. Translation(0)
- 12. Gene Regulation in Prokaryotes(0)
- 13. Gene Regulation in Eukaryotes(0)
- 14. Genetic Control of Development(0)
- 15. Genomes and Genomics(0)
- 16. Transposable Elements(0)
- 17. Mutation, Repair, and Recombination(0)
- 18. Molecular Genetic Tools(0)
- 19. Cancer Genetics(0)
- 20. Quantitative Genetics(0)
- 21. Population Genetics(0)
- 22. Evolutionary Genetics(0)
18. Molecular Genetic Tools
Genetic Cloning
18. Molecular Genetic Tools
Genetic Cloning: Study with Video Lessons, Practice Problems & Examples
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32PRACTICE PROBLEM
We need to calculate the minimum length of a primer for amplifying a specific gene in the pea (Pisum sativum) plant, assuming a desired melting temperature of 60°C, a GC content of 50%, and an equal frequency of all bases in the plant's genome, which is about 4.45 billion base pairs. Which of the following formulas to calculate the minimum primer length is correct?
We need to calculate the minimum length of a primer for amplifying a specific gene in the pea (Pisum sativum) plant, assuming a desired melting temperature of 60°C, a GC content of 50%, and an equal frequency of all bases in the plant's genome, which is about 4.45 billion base pairs. Which of the following formulas to calculate the minimum primer length is correct?