Table of contents
- 1. Introduction to Genetics(0)
- 2. Mendel's Laws of Inheritance(0)
- 3. Extensions to Mendelian Inheritance(0)
- 4. Genetic Mapping and Linkage(0)
- 5. Genetics of Bacteria and Viruses(0)
- 6. Chromosomal Variation(0)
- 7. DNA and Chromosome Structure(0)
- 8. DNA Replication(0)
- 9. Mitosis and Meiosis(0)
- 10. Transcription(0)
- 11. Translation(0)
- 12. Gene Regulation in Prokaryotes(0)
- 13. Gene Regulation in Eukaryotes(0)
- 14. Genetic Control of Development(0)
- 15. Genomes and Genomics(0)
- 16. Transposable Elements(0)
- 17. Mutation, Repair, and Recombination(0)
- 18. Molecular Genetic Tools(0)
- 19. Cancer Genetics(0)
- 20. Quantitative Genetics(0)
- 21. Population Genetics(0)
- 22. Evolutionary Genetics(0)
12. Gene Regulation in Prokaryotes
Lac Operon
12. Gene Regulation in Prokaryotes
Lac Operon: Study with Video Lessons, Practice Problems & Examples
Back to all problems
5PRACTICE PROBLEM
Suppose a researcher is studying the regulation of the lac operon in E. coli and performs a northern blot analysis. They isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype (I⁺ P⁺ O⁺ Z⁺ Y⁺) and another with a deletion mutation in the lacI gene. The researcher hybridizes the mRNA with a probe specific to the lacZ gene. Suppose the researcher includes a sample from an E. coli strain with a deletion mutation in the lacZ gene in the northern blot analysis. How would this affect the results of the hybridization?
Suppose a researcher is studying the regulation of the lac operon in E. coli and performs a northern blot analysis. They isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype (I⁺ P⁺ O⁺ Z⁺ Y⁺) and another with a deletion mutation in the lacI gene. The researcher hybridizes the mRNA with a probe specific to the lacZ gene. Suppose the researcher includes a sample from an E. coli strain with a deletion mutation in the lacZ gene in the northern blot analysis. How would this affect the results of the hybridization?