Table of contents
- 1. Introduction to Genetics(0)
- 2. Mendel's Laws of Inheritance(0)
- 3. Extensions to Mendelian Inheritance(0)
- 4. Genetic Mapping and Linkage(0)
- 5. Genetics of Bacteria and Viruses(0)
- 6. Chromosomal Variation(0)
- 7. DNA and Chromosome Structure(0)
- 8. DNA Replication(0)
- 9. Mitosis and Meiosis(0)
- 10. Transcription(0)
- 11. Translation(0)
- 12. Gene Regulation in Prokaryotes(0)
- 13. Gene Regulation in Eukaryotes(0)
- 14. Genetic Control of Development(0)
- 15. Genomes and Genomics(0)
- 16. Transposable Elements(0)
- 17. Mutation, Repair, and Recombination(0)
- 18. Molecular Genetic Tools(0)
- 19. Cancer Genetics(0)
- 20. Quantitative Genetics(0)
- 21. Population Genetics(0)
- 22. Evolutionary Genetics(0)
5. Genetics of Bacteria and Viruses
Bacterial Transformation
5. Genetics of Bacteria and Viruses
Bacterial Transformation: Study with Video Lessons, Practice Problems & Examples
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1PRACTICE PROBLEM
The researcher has a bacterial colony containing 500 cells that are a mixture of wild-type and auxotrophic mutants. To identify the auxotrophic mutants, the researcher performs replica plating. The colony is streaked onto a complete medium and allowed to grow. Afterward, a sterile velvet pad is placed onto the bacterial colony, picking up some of the bacterial cells. The pad is then used to make a replica of the bacterial colony onto two different media: minimal media and minimal media supplemented with the nutrient that the auxotrophic mutants require.
What is the purpose of replica plating in identifying auxotrophic mutants in a bacterial colony?
The researcher has a bacterial colony containing 500 cells that are a mixture of wild-type and auxotrophic mutants. To identify the auxotrophic mutants, the researcher performs replica plating. The colony is streaked onto a complete medium and allowed to grow. Afterward, a sterile velvet pad is placed onto the bacterial colony, picking up some of the bacterial cells. The pad is then used to make a replica of the bacterial colony onto two different media: minimal media and minimal media supplemented with the nutrient that the auxotrophic mutants require.
What is the purpose of replica plating in identifying auxotrophic mutants in a bacterial colony?