Table of contents
- 1. Introduction to Genetics(66)
- 2. Mendel's Laws of Inheritance(333)
- 3. Extensions to Mendelian Inheritance(204)
- 4. Genetic Mapping and Linkage(147)
- 5. Genetics of Bacteria and Viruses(101)
- 6. Chromosomal Variation(106)
- 7. DNA and Chromosome Structure(129)
- 8. DNA Replication(69)
- 9. Mitosis and Meiosis(67)
- 10. Transcription(89)
- 11. Translation(150)
- 12. Gene Regulation in Prokaryotes(102)
- 13. Gene Regulation in Eukaryotes(124)
- 14. Genetic Control of Development(76)
- 15. Genomes and Genomics(138)
- 16. Transposable Elements(26)
- 17. Mutation, Repair, and Recombination(148)
- 18. Molecular Genetic Tools(146)
- 19. Cancer Genetics(62)
- 20. Quantitative Genetics(96)
- 21. Population Genetics(112)
- 22. Evolutionary Genetics(38)
12. Gene Regulation in Prokaryotes
Lac Operon
12. Gene Regulation in Prokaryotes
Lac Operon: Videos & Practice Problems
6PRACTICE PROBLEM
Suppose that you are performing a northern blot analysis to study the expression of the lac operon in E. coli strains, and you isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype (I⁺ P⁺ O⁺ Z⁺ Y⁺) and another with a lac⁻ genotype (I⁺ P⁻ Oᶜ Z⁻ Y⁻). The probe used in the northern blot analysis hybridizes to a portion of the lacZ sequence.
What will result from the lacZ probe's hybridization for the mutant strain?
Suppose that you are performing a northern blot analysis to study the expression of the lac operon in E. coli strains, and you isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype (I⁺ P⁺ O⁺ Z⁺ Y⁺) and another with a lac⁻ genotype (I⁺ P⁻ Oᶜ Z⁻ Y⁻). The probe used in the northern blot analysis hybridizes to a portion of the lacZ sequence.
What will result from the lacZ probe's hybridization for the mutant strain?