A 3.5-kb segment of DNA containing the complete sequence of a mou...

Question

A 3.5-kb segment of DNA containing the complete sequence of a mouse gene is available. The DNA segment contains the promoter sequence and extends beyond the polyadenylation site of the gene. The DNA is studied by band shift assay (see Research Technique 8.1), and the following gel bands are observed. Match these conditions to a specific lane of the gel. 3.5-kb fragment plus RNA polymerase II

Accepted Answer

Welcome back, everyone. Let's look at our next problem. The band shift essay was used to examine a segment of DNA containing the whole sequence of a gene, including the promoter and terminator regions. The following image shows the gel bands that were observed and we have an image of Joe with five lanes and bands of five different sizes. And then our question says, determine which of the following lanes is most likely to correspond to naked DNA plus R N A polymerase two plus all general transcription factors choice A lane two, B lane three C lane four or D lane five. Well, we want to remember on bands shift essays that the more proteins are bound to the DNA, the lower the mobility. So that means that the band will be higher on the gel since it can't travel as far. And it's asking us which lane is most likely to correspond to DNA bound to R N A polymerase and all the general transcription factors. So we would expect this to be the largest slash highest band because it has the most possible things bound to the DNA. So when we look at our gel, we see that Lane two has the largest band. It's the highest up of all the bands on the show. So that would correspond to choice A here. So our answer to which band will correspond to that DNA plus R N A Plimer plus all the general transcription factors in a band shift assay gel is choice A Lane two. See you in the next video.

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