While circular RNAs were first described long ago, they ...

Question

While circular RNAs were first described long ago, they have only recently been investigated for function. What are their known and suspected functions in the cell?

Accepted Answer

Hi everyone. Let's look at our next problem, identify the incorrect statement about circular RNA. Let's look through our answer choices. Keeping in mind that we're looking for an incorrect statement. Also note that choice D here is all of the above. There is that potential for all of our answers. Being correct. Choice says these are RNA molecules with a circular structure. Well, this is correct and therefore not our answer choice. Across that out there. Circular RNA as their name suggests, are just pieces of RNA that rather than being linear are wrapped around with the head and tail together to make a circular piece of RNA choice B says they are involved in post transcription and gene regulation. Well, this is another correct statement and therefore not our answer choice. Uh They are have played a role in post transcription. AlS gene regulation and gene expression. So we know also the choice D cannot be correct since we now have to answer choices that are not our answer. So we have choice C left. They can be detected by using northern blotting Well, a northern blot is not the best way to take this circular DNA. Excuse me, circular RNA. Uh Northern boat is much, much less sensitive tool. It only can detect the most abundant circular RNA is. So the most preferred technique for detecting these is actually our T. PCR or reverse transcriptase PCR which can look for the specific what's called back splicing junction where you have the two ends of the RNA molecule meet to create a circle and the RT PCR is more sensitive for finding those back splicing junction that let you know this is a circular RNA, so there's our answer choice. The incorrect statement about circular RNA is choice C. They can be detected by using northern blotting, See you in the next video.

While circular RNAs were first described long ago, they have only recently been investigated for function. What are their known and suspected functions in the cell?

Key Concepts

Circular RNAs (circRNAs)

Functions of circRNAs

Biological significance of circRNAs

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