18. Molecular Genetic Tools
Genetic Cloning
1:53 minutes
Problem 31a
Textbook Question
Textbook QuestionYou have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temperatures. You wish to transfer this gene into E. coli to produce industrial amounts of enzyme for use in laundry detergent. You have managed to produce transgenic E. coli expressing mRNA of your gene, but only a low level of protein is produced. Why might this be so? How could you overcome this problem?
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