You have cloned a gene for an enzyme that degrades lipids in a ba...

Question

You have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temperatures. You wish to transfer this gene into E. coli to produce industrial amounts of enzyme for use in laundry detergent.

You have managed to produce transgenic E. coli expressing mRNA of your gene, but only a low level of protein is produced. Why might this be so? How could you overcome this problem?

Accepted Answer

Hey, everyone. Let's take a look at this question together in the history of insulin production, insulin was harvested from bovine and swine. However, due to side effects, researchers considered experimenting with bacteria, they introduced functional human insulin genes into the bacteria, creating a transgenic organism, which of the following bacteria were used in this process. Is it answer choice. A ecoli is it answer choice Bs Arius, is it answer choice C K pneumonia or answer choice D V cholera. Let's work this problem out together to try to figure out which of the falling bacteria was the bacteria that was used in the transgenic organism process. So in order to create that transgenic organism in which they introduce the functional human insulin genes into to the bacteria, they required a specific type of bacteria. And so their perfect bacteria to use in this process had a high growth rate, had a simple medium requirement, was very cost effective, was easy to handle and it was not too sensitive to changes in environmental conditions. And so the type of bacteria that met all of these requirements in order to create that transgenic organism was the bacteria known as e coli. So answer choice. A is the correct answer since E coli had a high growth rate, simple medium requirement was very cost effective, was easy to handle and was not too sensitive to changes in environmental conditions which allowed them to introduce those functional human insulin genes into the E coli and create that transgenic organism. I hope you found this video to be helpful. Thank you and goodbye.

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