Hemoglobins from two individuals are compared by electrophoresis ...

Question

Hemoglobins from two individuals are compared by electrophoresis and by fingerprinting. Electrophoresis reveals no difference in migration, but fingerprinting shows an amino acid difference. How is this possible?

Accepted Answer

Hello everyone and welcome to today's video. So the proteins are separated on the basis of electrophrenic mobility by well, let's go over each of our answer choices so that we may the term the correct one. So first we have affinity chromatography in affinity chromatography, certain molecules are going to be separated from each other based on affinity, two certain compounds. This is not what we're looking for. So we're going to cancel it out. Then we have C which is the ion exchange chromatography here, ions and polar molecules are going to be separated based on their affinity to ion exchangers. Also know what we're looking for because the question states that we're looking for something that separates them on the basis of electrophrenic mobility. So we're going to also cancel and choice C out. Then we have D which is dialysis. Well, dialysis is going to separate very small molecules based on their ability to pass via a semi permeable membrane. Again, not based on electrolytic mobility. So we're going to cancel it out. And now we have B which is SDS page or SDS page is a very effective technique to separate proteins. And it does So based on the protein's affinity to an electrode, which is what the question means when they state electro mobility and therefore answer choice A B sds page is going to be the correct answer for this question. And we're going to highlight it. I really hope this video helped you and I hope to see you on the next one.

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Hemoglobins from two individuals are compared by electrophoresis and by fingerprinting. Electrophoresis reveals no difference in migration, but fingerprinting shows an amino acid difference. How is this possible?