Using the genomic libraries in Problem 4, you wish to clone the h...

Question

Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

How frequently will a clone representing myostatin be found in the cDNA library made from brain?

Accepted Answer

Hi, everyone. Let's take a look at this practice problem together. Which statement below does not accurately describe the differences between a genomic library and AC DNA library. So first let's review what we know about genomic and C DNA libraries and then we'll go back through together and read the answer options. So I wanna begin by discussing genomic libraries. Now recall that genomic libraries are obtained using DNA as the starting material. That means that it represents the entire genome because it represents the entire genome. Genomic libraries contain both coding and noncoding regions and thus they are often larger than C DNA libraries. Now, C DNA libraries recall that C DNA is complimentary DNA and the starting material for C DNA libraries is what it uses MRN A. So you have to have trans reverse transcription to occur to obtain the C DNA. Now, since the starting material is MRN AC DNA libraries only contain the coding regions and thus they are often smaller than the genomic libraries. So now that we've reviewed that, let's go ahead and read through each answer option, option. A genomic libraries contain both coding and noncoding regions of DNA. While C DNA libraries only contain coding regions. So this is true. That's a true statement we just reviewed that. All right. Now, option B is genomic libraries are constructed using MRN A while C DNA libraries are constructed using genomic DNA. So this is not true. Genomic libraries are constructed using DNA and C DNA libraries use MRN A. So option B is a false statement. Option C genomic libraries can represent the entire genome of an organism while C DNA libraries only represent the genes that are actively transcribed. This is also a true statement. Option D the DNA in gene in genomic libraries is fragmented and cloned into vectors. While the DNA in C DNA libraries is reverse transcribed into C DNA and then cloned into vectors. And that is also true. Remember the MRN A has to be revered transcribed to make the C DNA. So option D is also true. That means the statement that is not accurately describing the differences is option B. So the correct answer is option B, genomic libraries are constructed using MRN A while C DNA libraries are constructed using genomic DNA. All right, everyone. I hope you found this helpful and I'll see you soon for the next practice problem.

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Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells. How frequently will a clone representing myostatin be found in the cDNA library made from brain?