18. Molecular Genetic Tools
Genetic Cloning
2:57 minutes
Problem 16
Textbook Question
Textbook QuestionThe restriction enzymes XhoI and SalI cut their specific sequences as shown below:
Xhol 5'-C TCGAG-3'
3'-GAGCT C-5'
Sall 5'-G TCGAC-3'
3'-CAGCT G-5'
Can the sticky ends created by XhoI and SalI sites be ligated? If yes, can the resulting sequences be cleaved by either XhoI or SalI?
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