18. Molecular Genetic Tools
Methods for Analyzing DNA
3:24 minutes
Problem 28b
Textbook Question
Textbook QuestionIn a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated material for DNA sequencing gels. Reaction products are usually run in gel lanes labeled A, T, C, and G.
Why is incorporation of a dideoxynucleotide during DNA sequencing identified as a 'replication-terminating' event?
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