Define and distinguish epistasis and pleiotropy.

A researcher interested in studying a human gene on chromosome 21 and another gene on the X chromosome uses FISH probes to locate each gene. The chromosome 21 probe produces green fluorescent color, and the X chromosome probe produces red fluorescent color.

If the subject studied is male, how many green and red spots will be detected? Explain your answer.

A researcher interested in studying a human gene on chromosome 21 and another gene on the X chromosome uses FISH probes to locate each gene. The chromosome 21 probe produces green fluorescent color, and the X chromosome probe produces red fluorescent color.

If the subject studied is female, how many green and red spots will be detected? Explain your answer.

In what way does position effect variegation (PEV) of Drosophila eye color indicate that chromatin state can affect gene transcription?

Strains of petunias come in four pure-breeding colors: white, blue, red, and purple. White petunias are produced when plants synthesize no flower pigment. Blue petunias and red petunias are produced when plants synthesize blue or red pigment only. Purple petunias are produced in plants that synthesize both red and blue pigment (the mixture of red and blue makes purple). Flower-color pigments are synthesized by gene action in two separate pigment-producing biochemical pathways. Pathway I contains gene A that produces an enzyme to catalyze conversion of a colorless pigment designated  to blue pigment. In Pathway II, the enzymatic product of gene B converts the colorless pigment designated  to red pigment. The two genes assort independently.

What are the possible genotype(s) for true-breeding blue petunias? 

Strains of petunias come in four pure-breeding colors: white, blue, red, and purple. White petunias are produced when plants synthesize no flower pigment. Blue petunias and red petunias are produced when plants synthesize blue or red pigment only. Purple petunias are produced in plants that synthesize both red and blue pigment (the mixture of red and blue makes purple). Flower-color pigments are synthesized by gene action in two separate pigment-producing biochemical pathways. Pathway I contains gene A that produces an enzyme to catalyze conversion of a colorless pigment designated  to blue pigment. In Pathway II, the enzymatic product of gene B converts the colorless pigment designated  to red pigment. The two genes assort independently.

What are the possible genotype(s) for pure-breeding red petunias? 

Feather color in parakeets is produced by the blending of pigments from two biosynthetic pathways shown below. Four independently assorting genes (A, B, C, and D) produce enzymes that catalyze separate steps of the pathways. For the questions below, use an uppercase letter to indicate a dominant allele producing full enzymatic activity and a lowercase letter to indicate a recessive allele producing no functional enzyme. Feather colors produced by mixing pigments are green (yellow + blue) and purple (red + blue). Red, yellow, and blue feathers result from production of one colored pigment, and white results from absence of pigment production.

What is the genotype of a pure-breeding purple parakeet strain? 

Feather color in parakeets is produced by the blending of pigments from two biosynthetic pathways shown below. Four independently assorting genes (A, B, C, and D) produce enzymes that catalyze separate steps of the pathways. For the questions below, use an uppercase letter to indicate a dominant allele producing full enzymatic activity and a lowercase letter to indicate a recessive allele producing no functional enzyme. Feather colors produced by mixing pigments are green (yellow + blue) and purple (red + blue). Red, yellow, and blue feathers result from production of one colored pigment, and white results from absence of pigment production.

What is the genotype of a pure-breeding yellow strain of parakeet? 

Feather color in parakeets is produced by the blending of pigments from two biosynthetic pathways shown below. Four independently assorting genes (A, B, C, and D) produce enzymes that catalyze separate steps of the pathways. For the questions below, use an uppercase letter to indicate a dominant allele producing full enzymatic activity and a lowercase letter to indicate a recessive allele producing no functional enzyme. Feather colors produced by mixing pigments are green (yellow + blue) and purple (red + blue). Red, yellow, and blue feathers result from production of one colored pigment, and white results from absence of pigment production.

If F₁ birds identified in part (c) are mated at random, what phenotypes do you expect in the F₂ generation? What are the ratios among phenotypes? Show your work. 

A male and a female mouse are each from pure-breeding albino strains. They have a litter of 10 pups, all of which have normal pigmentation. The F₁ pups are crossed to one another to produce 56 F₂ mice, of which 31 are normally pigmented and 25 are albino.

What genetic phenomenon explains the F₂ results? Use your allelic symbols to explain the F₂ results.

A male and a female mouse are each from pure-breeding albino strains. They have a litter of 10 pups, all of which have normal pigmentation. The F₁ pups are crossed to one another to produce 56 F₂ mice, of which 31 are normally pigmented and 25 are albino.

Using clearly defined allele symbols of your own choosing, give the genotypes of parental and F₁ mice. What genetic phenomenon explains these parental and F₁ phenotypes?

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

What proportion of the F₂ will have yellow seeds? Show your work. 

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

If strains G₂ and G₃ are crossed, what will be the phenotype of the F₁? 

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

What proportion of the F₂ are expected to be green? Show your work. 

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

If green-seeded strains G₁ and G₃ are crossed, what are the phenotype and the genotype of F₁ progeny? 

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

For each set of F₂ progeny, provide a genetic explanation for the yellow : green ratio. What are the genotypes of yellow and green F₂ lentil plants in the G₂ x Y cross? 

Three strains of green-seeded lentil plants appear to have the same phenotype. The strains are designated G₁, G₂, and G₃. Each green-seeded strain is crossed to a pure-breeding yellow-seeded strain designated Y. The F₁ of each cross are yellow; however, self-fertilization of F₁ plants produces F₂ with different proportions of yellow- and green-seeded plants as shown below.

For what number of genes are variable alleles segregating in the G₁ x Y cross? The G₂ x Y cross? In the G₃ x Y cross? Explain your rationale for each answer. 

Blue flower color is produced in a species of morning glories when dominant alleles are present at two gene loci, A and B. (Plants with the genotype  have blue flowers.) Purple flowers result when a dominant allele is present at only one of the two gene loci, A or B. (Plants with the genotypes  and  are purple.) Flowers are red when the plant is homozygous recessive for each gene (i.e., aabb).

Two pure-breeding purple strains are crossed, and all the F₁ plants have blue flowers. What are the genotypes of the parental plants?

Blue flower color is produced in a species of morning glories when dominant alleles are present at two gene loci, A and B. (Plants with the genotype  have blue flowers.) Purple flowers result when a dominant allele is present at only one of the two gene loci, A or B. (Plants with the genotypes  and  are purple.) Flowers are red when the plant is homozygous recessive for each gene (i.e., aabb).

If two F₁ plants are crossed, what are the expected phenotypes and frequencies in the F₂?

Blue flower color is produced in a species of morning glories when dominant alleles are present at two gene loci, A and B. (Plants with the genotype  have blue flowers.) Purple flowers result when a dominant allele is present at only one of the two gene loci, A or B. (Plants with the genotypes  and  are purple.) Flowers are red when the plant is homozygous recessive for each gene (i.e., aabb).

If an F₁ plant is backcrossed to one of the pure-breeding parental plants, what is the expected ratio of phenotypes among progeny? Why is the phenotype ratio the same regardless of which parental strain is selected for the backcross?

The crosses shown are performed between morning glories whose flower color is determined as described in Problem 24. Use the segregation data to determine the genotype of each parental plant.

Two pure-breeding strains of summer squash producing yellow fruit, Y₁ and Y₂, are each crossed to a pure-breeding strain of summer squash producing green fruit, G₁, and to one another. The following results are obtained:

Examine the results of each cross and predict how many genes are responsible for fruit-color determination in summer squash. Justify your answer. 

Two pure-breeding strains of summer squash producing yellow fruit, Y₁ and Y₂, are each crossed to a pure-breeding strain of summer squash producing green fruit, G₁, and to one another. The following results are obtained:

Using clearly defined symbols of your choice, give the genotypes of parental, F₁, and F₂  plants in each cross. 

Two pure-breeding strains of summer squash producing yellow fruit, Y₁ and Y₂, are each crossed to a pure-breeding strain of summer squash producing green fruit, G₁, and to one another. The following results are obtained:

If the F₁ of Crosses I and II are mated, predict the phenotype ratio of the progeny. 

Human ABO blood type is determined by three alleles, two of which (I^A and I^B) produce gene products that modify the H antigen produced by protein activity of an independently assorting H gene. A rare abnormality known as the 'Bombay phenotype' is the result of epistatic interaction between the gene for the ABO blood group and the H gene. Individuals with the Bombay phenotype appear to have blood type O based on the inability of both anti-A antibody and anti-B antibody to detect an antigen. The apparent blood type O in Bombay phenotype is due to the absence of H antigen as a result of homozygous recessive mutations of the H gene. Individuals with the Bombay phenotype have the hh genotype. Use the information above to make predictions about the outcome of the cross shown below.

IᴬIᴮHh×IᴬIᴮHh

Epistatic gene interaction results in a modification of the F₂ dihybrid ratio.

Give two examples of modified F₂ ratios produced by epistatic gene interactions and describe how gene interaction results in the ratios.

Epistatic gene interaction results in a modification of the F₂ dihybrid ratio.

What genetic principle is the basis of this expected F₂ ratio?

Epistatic gene interaction results in a modification of the F₂ dihybrid ratio.

What is the expected F₂ ratio?

When performing a complementation test, how do you know if two mutations complement?

How can you tell if two genes are epistatic?

Two heterozygous organisms are crossed, and the F₂ phenotypic ratio is 12:3:1. What form of epistasis do these two genes exhibit?

A cross of white plants and red plants was performed. Using the F2 phenotypic ratio data below, determine what form of gene interaction is taking place. 

In the rare Bombay phenotype, a mutation in a second gene can control an individual's what?