Substantial fractions of the genomes of many plants consist of se...

Question

Substantial fractions of the genomes of many plants consist of segmental duplications; for example, approximately 40% of genes in the Arabidopsis genome are duplicated. How might you approach the functional characterization of such genes using reverse genetics?

Accepted Answer

Hello, everyone and welcome to today's video. So which of the following involves disrupting a gene's sequence to analyze its function. Now, let's go over each of our answer choices so that we may solve the problem beginning by answer choice eight, which is the two hybrid test. Well, this two Highbridge test is going to be used to analyze protein interactions within lives cells is not going to help us disrupt the gene sequence. So we're going to cancel it out. Then we have B which is chromatin immunoprecipitation also called she. Now she is a technique that is going to help us analyze or study DNA to protein interactions to study things like the binding site in DNA of certain transcription factors or his. Again, this is not going to help us to disrupt the gene sequence. So we're going to cancel it out. Then we have D which is DNA micro arrays. Remember from previous videos that DNA microarray is a technique used to study which genes are going to be expressed under certain circumstances. An example of this is that we can study which genes are going to be expressed under the cancer using DNA micro or rays, but this is not going to help us disrupt the gene sequence. So we're going to cancel it out. And finally, we have C which is reverse genetics. Now, reverse genetics is a technique that is going to use things like points or other mutations to disrupt a genes sequence so that we may then analyze its function obtained by DNA sequencing. Therefore, this is going to be our correct answer choice. So answer choice c reverse genetics is a final answer to this question. I really hope this helped you and I hope to see you on the next video.

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Substantial fractions of the genomes of many plants consist of segmental duplications; for example, approximately 40% of genes in the Arabidopsis genome are duplicated. How might you approach the functional characterization of such genes using reverse genetics?