Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
13. Gene Regulation in Eukaryotes
Overview of Eukaryotic Gene Regulation
Problem 2e
Textbook Question
Describe and give an example (real or hypothetical) of each of the following:
RNA interference
![](/channels/images/assetPage/verifiedSolution.png)
1
Step 1: Understand the concept of RNA interference (RNAi). RNA interference is a biological process in which RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA molecules. It is a crucial mechanism for regulating gene expression and defending against viral genomes.
Step 2: Identify the key components involved in RNA interference. The main components include small interfering RNA (siRNA) or microRNA (miRNA), the RNA-induced silencing complex (RISC), and the target mRNA. siRNA or miRNA are short RNA molecules that guide the RISC to the complementary mRNA.
Step 3: Describe the process of RNA interference. The process begins with the introduction of double-stranded RNA (dsRNA) into the cell, which is then processed into siRNA by the enzyme Dicer. The siRNA is incorporated into the RISC, which uses one strand of the siRNA to bind to the complementary sequence on the target mRNA, leading to its degradation or translational repression.
Step 4: Provide a real or hypothetical example of RNA interference. A real-world example is the use of RNAi in research to silence specific genes in model organisms like C. elegans or Drosophila to study gene function. A hypothetical example could be using RNAi to silence a gene responsible for a genetic disorder in humans, potentially reducing the symptoms or progression of the disease.
Step 5: Discuss the significance of RNA interference in research and medicine. RNAi has become a powerful tool in functional genomics, allowing researchers to study the effects of gene silencing. It also holds potential therapeutic applications, such as developing RNAi-based drugs to target and silence disease-causing genes.
Recommended similar problem, with video answer:
![](/channels/images/assetPage/verifiedSolution.png)
This video solution was recommended by our tutors as helpful for the problem above
Video duration:
3mPlay a video:
Was this helpful?
Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
RNA Interference (RNAi)
RNA interference is a biological process in which RNA molecules inhibit gene expression or translation, effectively silencing targeted genes. This mechanism is crucial for regulating gene activity and defending against viral infections. RNAi is mediated by small RNA molecules, such as small interfering RNA (siRNA) and microRNA (miRNA), which bind to complementary mRNA sequences, leading to their degradation or preventing their translation.
Recommended video:
Guided course
RNA Interference
Mechanism of Action
The mechanism of RNA interference involves the processing of double-stranded RNA (dsRNA) into small RNA fragments by an enzyme called Dicer. These small RNA molecules then associate with a protein complex known as the RNA-induced silencing complex (RISC). The RISC uses the small RNA as a guide to recognize and bind to complementary mRNA, resulting in either the cleavage of the mRNA or the inhibition of its translation, thereby silencing the gene.
Recommended video:
Guided course
Other Gene Interactions
Applications of RNAi
RNA interference has significant applications in research and medicine, particularly in gene silencing and therapeutic interventions. For example, scientists can use RNAi to knock down the expression of specific genes to study their function or to develop treatments for diseases such as cancer and viral infections. Hypothetically, a therapeutic RNAi could be designed to target and silence a gene responsible for tumor growth, potentially halting cancer progression.
Recommended video:
Guided course
Artificial Selection
Watch next
Master Review of Regulation with a bite sized video explanation from Kylia Goodner
Start learningRelated Videos
Related Practice