Devoting a few sentences to each, describe the following struc...

Question

Devoting a few sentences to each, describe the following structures or complexes and their effects on eukaryotic gene expression: Dicer

Accepted Answer

Hi everybody. Here's our next question. Which one of the following subunits or domains of dyer is involved in the cleavage of double stranded R N A. And our answer choices are A P A Z domain B R N A three domain C helicase domain or D D E X D slash HBO R N A helicase domain. So let's recall what we know of dicer. It's a protein that cleaves R N A molecules into SI R N A which is small interfering R N A or micro R N A as part of the R N A interference pathway. So it generates these little double stranded R N A. So small double stranded R N A molecules that suppress gene expression by binding to specific sequences. Since their R N A molecules, they combine to specific DNA sequences and suppress or interfere with the expression of genes. So we have this dicer protein, it cleaves double stranded D double stranded R N A molecules into small SI R N A or micro R N A as part of this pathway. So let's look at our answer choices. Four different domain choices. We can go ahead and eliminate choice D that D E X DH box R N A helicase that is an R N A helicase that unwinds double stranded R N A. But it's not one of the domains of dicer, not part of the dicer protein. So let's look at our other answer choices and we know that the dicer is going to cleave double stranded R N A. And we're looking for the domain that does that, even if we couldn't recall right away, we're going to look at the R N A three domain, which is choice B knowing that an R N A would be something that cleaves R N A. So that will guide us to choice B, which is indeed our correct answer. Our other answer. Choices the P A Z domain, which is choice A, that's the domain that binds to the three prime end of that double strand of DNA to allow the cleavage to take place, but not the part that does the cleavage itself. And then to see the helicase domain again, that name can give us a clue. The helicase domain is what unwinds the double stranded R N A for cleavage should take place but not what does the cleavage itself. So again, the subunit of dicer that's involved in the cleavage of double stranded R N A is choice B the R N A S3 domain. See you in the next video

Devoting a few sentences to each, describe the following structures or complexes and their effects on eukaryotic gene expression: Dicer

Key Concepts

Dicer

RNA Interference (RNAi)

Gene Expression Regulation

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