In Genetic Analysis 14.1 we designed a screen to identify conditi...

Question

In Genetic Analysis 14.1 we designed a screen to identify conditional mutants of S. cerevisiae in which the secretory system was defective. Suppose we were successful in identifying 12 mutants.

Based on your knowledge of the genetic tools for studying baker's yeast, how would you clone the genes that are mutated in your respective yeast strains? What is an approach to cloning the human orthologs (see Genetic Analysis 14.2 for definition) of the yeast genes?

Accepted Answer

Hey, everyone. Let's take a look at this question together. Suppose you want to introduce a human DNA containing the gene of interest into a bacterial DNA, which of the following vectors can be used in your experiment. Is it answer choice. A plasmids, answer choice B viral vectors, answer choice C cosmid or answer choice D artificial chromosomes. Let's work this problem out together to try to figure out which of the following vectors can we use to introduce human DNA containing the gene of interest into bacterial DNA? So we want to determine of the falling vectors, which one can be used to introduce human DNA, which contains a gene of interest into a bacterial DNA. And we can recall that in bacterial cells that foreign DNA in this case is the human DNA can be introduced by using a vector that is called a plasmid. And we know that a plasmid is a small circular double stranded DNA molecule that are physically separated from the chromosomal DNA to undergo independent replication. So this plasmid can be used to introduce human DNA that contains a gene of interest into that bacterial DNA. So answer choice. A plasmids is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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