In humans, congenital heart disease is a common birth defect that affects approximately 1 out of 125 live births. Using reverse transcription PCR (RT-PCR) Samir Zaidi and colleagues [(2013) Nature 498:220.223] determined that approximately 10 percent of the cases resulted from point mutations, often involving histone function. To capture products of gene expression in developing hearts, they used oligo(dT) in their reverse transcription protocol. Compared with oligo(dT) primers, a pool of random sequence primers requires a trickier assessment of annealing temperature. Why?

Hello everyone here We have a question asking the ideal length of primary use in the preliminaries chain reaction is a Pcr. Primer should be between 10 and 20 nucleotides longs. Its length offers appropriate specifically and binding strength to the target DNA template while limiting non specific binding to other portions of the template or to the PCR reaction components. If it is shorter or longer, this can result in reduced pcr amplification efficiency or non specific amplification. And while constructing this primer, it's crucial to take the primers G. C content, secondary structure and melting temperature into account. So our answer here is B 10 - 20 nucleotides. Thank you for watching. Bye.