The DNA sequences shown below are from the promoter regions of...

Question

The DNA sequences shown below are from the promoter regions of six bacterial genes. In each case, the last nucleotide in the sequence (highlighted in blue) is the nucleotide that initiates transcription. Determine the consensus sequence for the Pribnow box from these sequences. <>

Accepted Answer

Welcome back. Here's our next question. It says which of the following statements is correct regarding the prib. Now sequence choice A, it is also known as the minus 10 sequence as it is located base pairs upstream of the transcription site and plays a crucial role in the initiation of transcription. Choice B. It is composed of six base pair sequence T A T A A T which is recognized and bound by the sigma factor of R N A polymerase choice crib. Now box is located at the minus 35 region of bacterial promoters and is composed of six base pair sequence of TT G AC A choice D, both A and B and choice E all of the above. So let's evaluate our answer choices. Noticing that we have a choice of all three A B and C being correct or just A and B. So we're talking about the prib now sequence, this is one of the two conserved regions in the promoters of bacterial cells. So, bacterial promoters and it's located in the minus 10 region. So when we look at choice A that tells us it's the minus 10 sequence as it's 10 base pairs upstream of the transcription site and is important in the initiation of transcription. So this is correct. I'm not going to select it as our answer because I have the possibility of more than one correct. So I just put a little dot There choice B says it is composed of a six space pair sequence T A T A A T recognized and bound by the Sigma factor of R N A polymerase. Well, this is also correct that is the correct consensus sequence of the rib. Now box and it is the Sigma factor of R N A polymerase that finds it and binds to it. So B is also correct. So we're just left looking at tri C saying prib now box is located in the minus 35 regions for the region with the sequence of TT G AC A. And this is not correct. As we mentioned in discussing choice A, the box is located at the minus 10 region and we saw that this is not its consensus sequence that's listed in choice B. So choice C is not our answer and therefore choice E all of the above is also incorrect, but we have our two correct answers in choice A and B describing that prob now sequence. So our correct statement about the prib now sequence is choice D both A and B. See you in the next video.

My Courses

Chemistry

Biology

Math

Physics

Business

Social Sciences

Programming

Product & Marketing

The DNA sequences shown below are from the promoter regions of six bacterial genes. In each case, the last nucleotide in the sequence (highlighted in blue) is the nucleotide that initiates transcription. Determine the consensus sequence for the Pribnow box from these sequences. <>