In Genetic Analysis 14.1 we designed a screen to identify conditi...

Question

In Genetic Analysis 14.1 we designed a screen to identify conditional mutants of S. cerevisiae in which the secretory system was defective. Suppose we were successful in identifying 12 mutants.

Describe the crosses you would perform to determine the number of different genes represented by the 12 mutations.

Accepted Answer

Hi, everybody. Let's look at our question. It says which of the following approaches can be used to discover genetic interactions. A recombinant DNA B modifier, screening C polymerase chain reaction or D CRISPR cas. So let's recall if we're looking for genetic interactions, we'll be looking for ways that one gene might affect the phenotype caused by another gene. So it might enhance or repress it, but it has an effect on the action of another gene. It modifies it. So how do you discover ways in which you might have these interactions between different genes? And the answer is choice B modifier screening which involves using different chromosomal deletions to look for effects on genes when different other genes are deleted. So you delete a gene and then see, does that have an effect on the phenotype caused by another known gene? Let's look at our other answer choices to see why they're not correct choice. A recombinant DNA, it's just DNA that's artificially made by combining different DNA or DNA from different organisms. So that's not our answer choice here. So we'll cross that out and enjoy c polymerase chain reaction. Just that technique we use to amplify short sequences of DNA. So now what we're looking for since that won't discover genetic interactions, it just helps us make more of a specific DNA sequence. And then choice D CRISPR has is a technique for gene editing. So going in and actually modifying a gene, so not our correct answer here. So again, the technique that can be used to discover genetic interactions, choice B modifier screening. See you in the next video.

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