Compare the control of gene regulation in eukaryotes and bacteria at the level of initiation of transcription. How do the regulatory mechanisms work? What are the similarities and differences in these two types of organisms in terms of the specific components of the regulatory mechanisms?

The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.

Are intron regions single stranded or double stranded? Why?

The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.

Label the introns.

The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.

Illustrate the R-loop structure that would be seen with electron microscopy.

Research indicates that promoters may fall into one of two classes: focused or dispersed. How do these classes differ, and which genes tend to be associated with each?

Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.

Many transcriptional activators are proteins with a DNA-binding domain (DBD) and an activation domain (AD). Explain how each domain contributes to transcriptional initiation. Would you expect repressors to also have each of these domains?

How do the ENCODE data vastly help determine which enhancers regulate which genes?