Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacterial Transformation
3:49 minutes
Problem 23
Textbook Question
Textbook QuestionIn Bacillus subtilis, linkage analysis of two mutant genes affecting the synthesis of two amino acids, tryptophan (trp₂⁻), and tyrosine (trp₁⁻), was performed using transformation. Examine the following data and draw all possible conclusions regarding linkage. What is the purpose of Part B of the experiment? [Reference: E. Nester, M. Schafer, and J. Lederberg (1963).] Donor DNA Recipient Cell Transformants No. trp⁺ tyr⁻ 196 A. trp₂⁺ tyr₁⁺ trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 328 trp⁺ tyr⁺ 367 trp₂⁺ tyr₁⁻ trp⁺ tyr⁻ 190 B. and trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 256 trp₂⁻ tyr₁⁺ trp⁺ tyr⁺ 2
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