DNA footprint protection (described in Research Technique 8.1) is...

Question

DNA footprint protection (described in Research Technique 8.1) is a method that determines whether proteins bind to a specific sample of DNA and thus protect part of the DNA from random enzymatic cleavage by DNase I. A 400-bp segment of cloned DNA is thought to contain a promoter. The cloned DNA is analyzed by DNA footprinting to help determine if it has the capacity to act as a promoter sequence. The accompanying gel has two lanes, each containing the cloned 400-bp DNA fragment treated with DNase I to randomly cleave unprotected DNA. Lane 1 is cloned DNA that was mixed with RNA polymerase II and several TFII transcription factors before exposure to DNase I. Lane 2 contains cloned DNA that was exposed only to DNase I. RNA pol II and TFIIs were not mixed with that DNA before adding DNase I.

Explain why this gel provides evidence that the cloned DNA may act as a promoter sequence.

Accepted Answer

Hey, everyone. Let's take a look at this question. Together. Restriction enzymes are the enzymes that cut DNA at specific sites and are used as a tool for the restriction of the bacterial plasmid in molecular biology techniques, which of the following restriction enzymes recognizes the palindromic site that runs from the five end to the three end containing the following nucleotides. Is it answer choice. A pin D three answer choice B A L U one, answer choice C eco R one or answer choice D bam H one. Let's work this problem out together to try to figure out which of the following restriction enzymes recognizes that palindromic sequence. So in order to solve this question, let's look at each of the sequences that the following restriction enzymes will recognize to determine which of the following restriction enzyme recognizes the following palindromic sequence. So starting off with Hin D three, Hin D three recognizes the following sequence that runs from the five N to the three N containing these nucleotides. And since it does not match the palindromic sequence that we are looking for. Answer choice A N D three can be eliminated and we can take a look at the next restriction enzyme sequence. And A L U one recognizes a four base long sequence. So immediately it can be eliminated. Since the sequence we are looking for contains six base pairs. So it is not A L U one. And next we have ECO R one which recognizes the following sequence that runs from the five N to the three N containing those nucleotides. And we can see that the sequence that the restriction enzyme eco R one recognizes as the one that we are looking for. So answer choice C Eco R one is the correct restriction enzyme. So it is the correct answer. And lastly, we know that it is not answer choice D bam H one, since bam H one recognizes the following sequence that runs from the five N to the three N containing those nucleotides. And it is not the same sequence as the one that we are looking for though ECO R one which is answer choice C is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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