You have identified five genes in S. cerevisiae that are induced ...

Question

You have identified five genes in S. cerevisiae that are induced when the yeast are grown in a high-salt (NaCl) medium. To study the potential roles of these genes in acclimation to growth in high-salt conditions, you wish to examine the phenotypes of loss- and gain-of-function alleles of each.

How would your answer differ if you were working with tomato plants instead of yeast?

Accepted Answer

Hello, everyone and welcome to today's video. So the F L C gene encodes a transcription factor that plays a crucial role in flowering time regulation in Arabidopsis. The F L C gene is expressed at high levels during the winter and acts as a repressor of flowering in the spring. The F O C re expression levels decrease allowing flowering to occur. So which of the following techniques would be ideal to study the effect of F O C loss of function on flowering time regulation. So as answer choice A, we have western block analysis as answer choice B, we have R N A sequencing as answer choice C. We have transgenic over expression. And as answer choice D, we have CRISPR genome editing. Well as it turns out the best way to study and the F O C or choose any loss or function mutations is to create what we call a knock, knock out or a knockdown of the F L C gene. And in order to create a knock how we're going to be needing to use CRISPR genome editing. Because CRISPR technology allows for the precise and specific genetic modification and a crisp pr mediated L C knockout or knockdown would enable researchers to study the effects of the gene's absence on flagging time regulation, which is exactly what we're going for here. So answer choice D CRISPR genome editing is going to be the final answer to our question. I really hope this helped you and I hope to see you on the next one.

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