How would you design a genetic screen to find genes involved in m...

Question

How would you design a genetic screen to find genes involved in meiosis?

Accepted Answer

Hello everyone and welcome to today's video. So which method can be used to induce random mutations in the genome of a moral organism. For a genetic screen. Focus on meis as answer choice. A we have the polymerase chain reaction or PC R as answer choice B. We have whole genome sequencing as answer choice C we have L methane sulfonate or ems treatment. And as answer choice, the we have the G knockout using CRISPR cas nine. Now let's analyze each answer choice so that we may solve this problem as answer choice. A like we said we have polymer chain reaction or PCR. Remember from previous videos that polymer chain reaction is a technique that is going to help us replicate a specific sequence of DNA many, many, many times. So that then we can use it for other genetic processes. However, this is not going to help us in these mutations in this DNA. So we're going to cancel it out, then we have the whole genome sequencing and this is a very straightforward answer. Remember that whole genome sequencing is a technique that is going to help us sequence a genome of an organism. So that we may obtain information on its genetics. Again, this is not going to help us in these mutations. So we're going to cancel it out. Then we have D which is gene knockout using CRISPR Cas nine. Now CRISPR Cas nine can induce mutations. But remember from previous videos that the mutations by CRISPR Cas Nine are going to be guided. So they're not going to be random. Although some random mutations may occur due to certain errors in the CRISPR Cas nine complex, these mutations are going to be guided to a certain sequence of DNA. And therefore, it will not be used to induce random mutations. So we're going to cancel this out. And finally, we have C which is ethyl methanol or E MA treatment. Now, this ems is going to be a muto or a molecule or a substance that can induce mutations and it can chemically modify DNA by cul base leading to the introduction of mutations in the DNA. So if we introduce this, we can have the organism undergo random mutations such as point mutations or small insertions or deletions in the random points of the DNA. Therefore, this is going to be the correct answer for these questions. We're going to highlight answer choice C ethyl methanol or ems treatment as a final answer to this question. I really hope this helped you and I hope to see you on the next video

How would you design a genetic screen to find genes involved in meiosis?

Key Concepts

Meiosis

Genetic Screening

Mutagenesis

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