Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Transduction
2:35 minutes
Problem 24a
Textbook Question
Textbook QuestionThe phage P1 is used as a generalized transducing phage in an experiment combining a donor strain of E. coli of genotype leu⁺ phe⁺ ala⁺ and a recipient strain that is leu⁻ phe⁻ ala⁻. In separate experiments, transductants are selected for leu⁺ (Experiment A), for ala⁺ (Experiment B), and for phe⁺ (Experiment C). Following selection, transductant genotypes for the unselected markers are identified. The selection experiment results below show the frequency of each genotype. Experiment A Experiment B Experiment C phe⁻ ala⁻ 26% leu⁻ ala⁻ 65% leu⁻ phe⁻ 71% phe⁺ ala⁻ 50% leu⁺ ala⁻. 48% leu⁺ phe⁻ 21% phe⁻ ala⁺ 19% leu⁻ ala⁺ 0% leu⁻ phe⁺ 0% phe⁺ ala⁺ 3% leu⁺ ala⁺ 4% leu⁺ phe⁺ 3% In Experiment B, why are there no transductants with the genotype leu⁻ ala⁺?
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