Genomic DNA from a mouse is isolated, fragmented, and de...

Question

Genomic DNA from a mouse is isolated, fragmented, and denatured into single strands. It is then mixed with mRNA isolated from the cytoplasm of mouse cells. The image represents an electron micrograph result showing the hybridization of single-stranded DNA and mRNA.

Which nucleic acid is indicated by the 'a' pointer? Justify your answer.

Accepted Answer

Hi, everyone. Welcome back. Here's our next question. Which of the following characteristics distinguishes M R N A from DNA in a hybridization experiment. And our answer choices are A M R N A is single stranded. While DNA is double stranded choice, B M R N A contains ribo sugar. While DNA contains deoxy ribo sugar to C M R N A contains uracil nucleotide. While DNA contains thymine nucleotide and D M R N A has a shorter nucleotide sequence than DNA. Well, in a hybridization experiment, M R and and DNA are denatured and then allowed to anneal with each other. And the molecules are observed visually via electron micrograph. So we have to think about how this experiment will be observed when we look at our answer choices because all of our answer choices actually are correct in describing a difference between M R N A and DNA. We have M R N A as single stranded while DNA is double stranded M R N A ribose, DNA, deoxyribose M R N A containing uracil. While DNA contains thymine M R N A being having a shorter nucleotide sequence. Since portions of the DNA are spliced out to create the M R N A. However, if we're looking at this visually, the electron myography, the only difference that will be distinguishable by that means is choice am R N A being single stranded while DNA would be double stranded in that double helix structure. The distinguish between the types of sugars mentioned in choice B between the U and T nucleotides and the overall nucleotide sequence length. In choice D, none of those will be visibly different between M R N A and DNA. While we can see the difference between the single stranded versus double stranded structure. So which of the following characteristics distinguishes M R N A from DNA in a hybridization experiment. Choice am R N A is single stranded while DNA is double stranded. See you in the next video.

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