Answer the following questions about the accompanying diagram.

Is the DNA nearest A the template strand or the coding strand? <>

Key Concepts

Template Strand vs. Coding Strand

In DNA, the template strand is the strand that is used as a guide for synthesizing RNA during transcription. The coding strand, also known as the sense strand, has the same sequence as the RNA produced, except that thymine (T) is replaced by uracil (U) in RNA. Understanding the distinction between these two strands is crucial for interpreting genetic information and the direction of transcription.

Directionality of DNA Strands

DNA strands have directionality, indicated by the 5' and 3' ends. The template strand is read in the 3' to 5' direction during transcription, allowing RNA polymerase to synthesize RNA in the 5' to 3' direction. Recognizing this directionality is essential for determining which strand is the template and which is the coding strand based on their orientation.

Transcription Process

Transcription is the process by which genetic information from DNA is copied into RNA. During this process, RNA polymerase binds to the promoter region of the gene and unwinds the DNA, using the template strand to synthesize a complementary RNA strand. Understanding the transcription process helps clarify the roles of the template and coding strands in gene expression.