18. Molecular Genetic Tools
Genetic Cloning
3:55 minutes
Problem 15b
Textbook Question
Textbook QuestionYou have recovered a cloned DNA segment from a vector and determine that the insert is 1300 bp in length. To characterize this cloned segment, you isolate the insert and decide to construct a restriction map. Using enzyme I and enzyme II, followed by gel electrophoresis, you determine the number and size of the fragments produced by enzymes I and II alone and in combination, as recorded in the following table. Construct a restriction map from these data, showing the positions of the restriction-enzyme cutting sites relative to one another and the distance between them in units of base pairs.
Enzyme Restriction Fragment Sizes (bp)
I 350, 950
II 200, 1100
I and II 150, 200, 950
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