The RAS gene encodes a signaling protein that hydrolyzes GTP to G...

Question

The RAS gene encodes a signaling protein that hydrolyzes GTP to GDP. When bound by GDP, the RAS protein is inactive, whereas when bound by GTP, RAS protein activates a target protein, resulting in stimulation of cells to actively grow and divide. As shown in the accompanying sequence, a single base-pair mutation results in a mutant protein that is constitutively active, leading to continual promotion of cell proliferation. Such mutations play a role in the formation of cancer. You have cloned the wild-type version of the mouse RAS gene and wish to create a mutant form to study its biological activity in vitro and in transgenic mice. Outline how you would proceed.

Accepted Answer

Hey, everyone. Let's take a look at this question together. A mouse has undergone genetic modification to express a foreign gene. One is a common method for creating transgenic mice. Is it answer choice. A particle bombardment method is it answer choice B electroporation method. Answer choice C gel electrophoresis or answer choice D vortex mixing method. Let's work this problem out together to try to figure out which of the following answer choices is a common method for creating transgenic mice. So first let's recall what the term transgenic means. And we note that it describes an organism that contains foreign DNA in which that foreign DNA is inserted into the mice. Well, we can eliminate answer choice C gel electrophoresis since we know that gel electrophoresis is a method that is used to separate DNA fragments based on their size and charge. So answer choice C is incorrect. And instead the method that is commonly used to insert DNA into the cells of living organisms such as plants or animals. And in this case, we're talking about the mice involves small particles that are coated with that DNA bombard the cells of the target organism. And in this case, that target organism is the mice and the DNA that coats the small particles is that foreign DNA? And this process describes the process of particle bombardment which is answer choice. A the correct answer. Since the particle bombardment method uses a device known as a particle cannon or gene gun to coat small particles such as gold or tungsten with the foreign DNA to insert them into that target organism. So answer choice A is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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