Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
12. Gene Regulation in Prokaryotes
Lac Operon
2:17 minutes
Problem 36
Textbook Question
Textbook QuestionFor the following lac operon partial diploids, determine whether the synthesis of lacZ mRNA is 'constitutive,' 'inducible,' or 'uninducible,' and indicate whether the partial diploid is or (able or not able to utilize lactose).
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Lac Operon
The lac operon is a set of genes in E. coli that are involved in the metabolism of lactose. It consists of structural genes (lacZ, lacY, and lacA) and regulatory elements that control their expression. The operon is typically off when lactose is absent and can be turned on (induced) in the presence of lactose, allowing the bacteria to utilize this sugar as an energy source.
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Constitutive, Inducible, and Uninducible Expression
Constitutive expression refers to genes that are continuously expressed regardless of environmental conditions. Inducible expression occurs when gene expression is turned on in response to specific stimuli, such as the presence of lactose for the lac operon. Uninducible expression means that the gene cannot be activated, even in the presence of the inducer, often due to mutations in regulatory elements.
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Penetrance and Expressivity
Partial Diploids
Partial diploids are bacterial cells that contain two copies of some genes, typically due to the introduction of a plasmid or a second chromosome segment. In the context of the lac operon, analyzing partial diploids helps determine how mutations in one copy of the operon affect the expression of lacZ mRNA and the ability of the bacteria to utilize lactose, providing insights into gene regulation mechanisms.
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