A substantial fraction of almost every genome sequenced consists ...

Question

A substantial fraction of almost every genome sequenced consists of genes that have no known function and that do not have sequence similarity to any genes with known function.

How would your approach change if the genes of unknown function were in the human genome?

Accepted Answer

Hello, everyone and welcome to today's video. So a researcher wants to determine which genes are active in thyroid cancer cells. This can be accomplished by employing a ship B DNA microarray C two hybrid test and the reverse genetics. Well, let's first of all, think about what we're doing in this problem. We have certain genes and we need to identify when they are expressed or just under which conditions they will be expressed. So this is what we're going to be doing in the problem. We need to realize or just identify which genes are going to be expressed under the conditions of thyroid cancer cells knowing this, let's go over each of our answer choices so that we may solve the problem. So as answer choice A we have chip which is short for chromatin immunoprecipitation. What this is used to identify or study protein to DNA interactions. This is not really going to help us with gene expression under certain circumstances. So we're going to cancel it out. Then we have C which is going to be the two hybrid test. This is used to study protein to protein interactions within life cells. But again, it's not going to help us to help us with genes. So we're going to cancel it out, then we have D which is reverse genetics or reverse genetics is going to disrupt a gene sequence to analyze its function. So it's going to basically induce or reverse a mutation so that we can analyze the function of a gene. So this is not what we're looking for again. So we're going to cancel it out and then we have B which is DNA micro arrays. So DNA micro arrays are used to determine which genes in an organism are going to be active under certain circumstances and the circumstance in this case, like we said, it is going to be through cancer cells. So DNA microarray can help us with this process and we're going to highlight it as a final answer to this question. So B DNA microarray will be our final answer. I really hope this helped you and I hope to see you on the next video.

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A substantial fraction of almost every genome sequenced consists of genes that have no known function and that do not have sequence similarity to any genes with known function. How would your approach change if the genes of unknown function were in the human genome?