When the whole-genome shotgun sequence of the Drosophila genome w...

Question

When the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs.

Why were there so many more contigs than scaffolds?

Accepted Answer

Hello everyone and welcome to today's video. So which of the following statements is not true about shotgun sequencing. As answer choice. A we have shotgun sequencing is a Hyro method for sequencing DNA. As answer choice B we have shotgun sequencing involves randomly fragmenting the DNA into small pieces. As answer choice C we have shotgun sequencing typically results in a small number of contacts or and scaffolds. And as answer to D we have shotgun sequencing can be used for the novel sequencing of genomes. Now, as we all know shotgun sequencing is going to be used to sequence certain genomes in a very efficient way by cutting up the DNA into fragments. Now, shotgun sequencing typically results in a large number of context and scuffles particularly for larger and more complex genomes. Now, while this is not a necessary drawback in and out of itself, it can complicate genome assembly and annotation when we are sequencing these genomes and may require additional efforts in order to sequencing to sequence them. But this statement, it creates a large number of contexts and scaffold is going to be what is wrong with our question because answer choice C states char and sequencing typically results in a small number of contexts and scaffolds which we know is incorrect. So we're going to highlight it as a final answer to our question. I really hope this video helped you and I hope to see you on the next one.

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When the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs. Why were there so many more contigs than scaffolds?