Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells. How frequently will a clone representing myostatin be found in the cDNA library made from muscle?

Key Concepts

cDNA Library

A cDNA library is a collection of complementary DNA (cDNA) sequences synthesized from messenger RNA (mRNA) transcripts. It represents the expressed genes in a specific tissue or cell type, allowing researchers to study gene expression. In this context, a cDNA library made from muscle cells would contain clones of genes that are actively expressed in muscle tissue, including the myostatin gene.

Gene Cloning

Gene cloning is the process of creating copies of a specific gene or DNA sequence. This involves isolating the gene of interest, inserting it into a vector (like a plasmid), and introducing it into host cells for replication. In the case of myostatin, cloning allows for the study of its function and regulation, as well as potential applications in muscle growth research.

Expression Frequency

Expression frequency refers to how often a particular gene is expressed in a given tissue or under specific conditions. In a cDNA library, the frequency of a clone representing myostatin will depend on its expression level in muscle cells. If myostatin is highly expressed, it is more likely to be found frequently in the library, whereas low-expression genes may be represented less often.