You have constructed four different libraries: a genomic library ...

Question

You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library.

Which of these would have the greatest diversity of sequences?

Accepted Answer

Hi, everybody. Let's take a look at this practice problem together which of the following libraries is expected to have the highest diversity of sequences. The answer options are a human liver C DNA library B genomic library made from the human liver, C human kidney C DNA library and D all of them will have equal diversity of sequences. So sensor answer options are C DNA library and or genomic library. Let's go ahead and compare and contrast them. So on the left hand side of the screen, we will be collecting information for the genomic libraries. And on the right hand side, we'll be keeping tally of the information for C DNA libraries. Now, first off, let's start by talking about the starting material for the libraries in genomic DNA. The starting material is DNA. Whereas in C DNA, the starting material material is MRN A. So to make C DNA reverse transcription of the MRN A has to occur to make C DNA. That means that genomic libraries contains DNA clones, whereas the C DNA libraries contain C DNA clones. So recall that DNA contains both codeine and noncoding sequences. So since genomic libraries are made using DNA as the starting material. That means genomic libraries will contain codeine and noncoding sequences. Conversely, MRN A is made up of only coding sequences. So C DNA libraries are going to contain only coding sequences. That means that the genomic library is much larger than C DNA libraries. So our genomic library is larger, the C DNA library is smaller. Thus genomic libraries are also more diverse since they are clones of the entire genome. That means the correct answer is option B, the genomic library made from the human liver. All right, everyone. I hope you found this helpful and I'll see you soon for the next practice problem.

Key Concepts

Genomic Library

cDNA Library

Tissue-Specific Expression

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