A 3.5-kb segment of DNA containing the complete sequence of a mouse gene is available. The DNA segment contains the promoter sequence and extends beyond the polyadenylation site of the gene. The DNA is studied by band shift assay (see Research Technique 8.1), and the following gel bands are observed. Match these conditions to a specific lane of the gel. 3.5-kb fragment alone

Hi, everyone. Let's look at the next question which of the following is examined using the band shift essay choice A DNA DNA interactions, BRN A DNA interactions, C protein DNA interactions or D protein protein interactions. Well, in a band shift essay, we can recall that we compare on a gel, a sample of naked DNA, just DNA by itself and then samples of DNA with various proteins and those would be proteins that interact with DNA or bind to it. So a band shift assay therefore is ex examines choice C protein DNA interactions. The more proteins are bound to the DNA, the less mobility that protein DNA complex will have and the less far the band will travel in a gel. So you can see how many proteins are bound to a specific DNA sequence by how far a band travels compared to the naked DNA by itself. So again, band shift asa choice C protein DNA interactions. See you on the next video.