Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
11. Translation
The Genetic Code
Problem 21a
Textbook Question
In an experiment to decipher the genetic code, a poly-AC mRNA (ACACACAC...) is synthesized. What pattern of amino acids would appear if this sequence were to be translated by a mechanism that reads the genetic code as
a quadruplet with overlaps?
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1
<span>Step 1: Understand the sequence and reading frame.</span> The mRNA sequence is poly-AC, which means it repeats the sequence 'ACACACAC...'. In this problem, the genetic code is read as quadruplets with overlaps, meaning each set of four nucleotides is read, and then the reading frame shifts by one nucleotide to read the next set of four nucleotides.
<span>Step 2: Identify the possible quadruplets.</span> Start by identifying the first few quadruplets in the sequence: 'ACAC', 'CACA', 'ACAC', 'CACA', and so on. Notice the overlap as each new quadruplet starts one nucleotide after the previous one.
<span>Step 3: Translate each quadruplet.</span> Use the genetic code to translate each quadruplet into an amino acid. Since the standard genetic code is based on triplets, you will need to consider how the quadruplet might be interpreted. One approach is to consider the first three nucleotides of the quadruplet as a codon and the fourth nucleotide as the start of the next codon.
<span>Step 4: Determine the pattern of amino acids.</span> As you translate each overlapping quadruplet, note the amino acids that are produced. Since the sequence is repetitive, you may find a repeating pattern of amino acids.
<span>Step 5: Analyze the repeating pattern.</span> Once you have translated several quadruplets, look for a repeating pattern in the amino acids. This pattern will represent the sequence of amino acids produced by the poly-AC mRNA when read as overlapping quadruplets.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Genetic Code
The genetic code is a set of rules that defines how the sequence of nucleotides in mRNA is translated into amino acids, the building blocks of proteins. It consists of codons, which are triplets of nucleotides, but in this case, we are considering a quadruplet reading frame. Each codon corresponds to a specific amino acid or a stop signal during protein synthesis.
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Quadruplet Codons
Quadruplet codons refer to a hypothetical reading frame where four nucleotides are read together as a single unit, rather than the standard triplet codons. This approach can lead to different amino acid sequences being produced from the same mRNA sequence, as the grouping of nucleotides changes the interpretation of the genetic code.
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Overlapping Reading Frames
Overlapping reading frames occur when the same nucleotide sequence can be read in multiple ways, allowing for different sets of codons to be formed from the same sequence. This can lead to the production of different proteins from a single mRNA strand, depending on how the reading frame is shifted or adjusted, which is particularly relevant when considering quadruplet codons.
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