Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
8. DNA Replication
Overview of DNA Replication
4:29 minutes
Problem 14c
Textbook Question
Textbook QuestionBacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.
If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell?
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
DNA Replication
DNA replication is the biological process by which a cell duplicates its DNA before cell division. This process involves unwinding the double helix and synthesizing new strands complementary to the original ones. Key enzymes, including DNA polymerases, play crucial roles in adding nucleotides to the growing DNA strand and ensuring fidelity in the replication process.
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Steps to DNA Replication
Functions of DNA Polymerase I and III
DNA polymerase I and DNA polymerase III are essential enzymes in E. coli DNA replication. DNA polymerase III is the primary enzyme responsible for synthesizing the new DNA strand during replication, while DNA polymerase I is involved in removing RNA primers and replacing them with DNA. Inactivation of DNA polymerase I would hinder the removal of these primers, leading to potential issues in DNA strand continuity.
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Functional Genomics
Mutations and Their Effects
Mutations are changes in the DNA sequence that can affect gene function and protein production. If DNA polymerase I is inactivated, the cell may still replicate its DNA using DNA polymerase III, but it would likely accumulate RNA primers in the lagging strand. This could lead to abnormalities such as incomplete DNA strands or increased susceptibility to DNA damage, as the cell would struggle to maintain genomic integrity.
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