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Ch. 40 - Water and Electrolyte Balance in Animals
Chapter 39, Problem 14

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs. The scientists also measured the activity of Na+/K+-ATPase, in the gills of the fish exposed to aluminum and compared it to that of the control fish. What do you suppose were their results? Explain.

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Step 1: Understand the role of Na+/K+-ATPase in fish. Na+/K+-ATPase is an enzyme found in the cell membranes of fish gills that helps maintain osmotic balance and proper ion gradients by pumping sodium (Na+) out of the cells and potassium (K+) into the cells.
Step 2: Recognize the impact of aluminum on fish. Aluminum exposure in aquatic environments can lead to physiological stress in fish, affecting their gill function, which is crucial for respiration and ion regulation.
Step 3: Hypothesize the effect of aluminum on Na+/K+-ATPase activity. Given the toxic nature of aluminum, it is likely that the activity of Na+/K+-ATPase in the gills of fish exposed to aluminum would be reduced. This reduction could impair ion regulation and osmotic balance in the fish.
Step 4: Compare the activity of Na+/K+-ATPase in exposed fish versus control fish. The scientists would measure and compare the enzyme activity levels. It is expected that the fish exposed to aluminum would show lower Na+/K+-ATPase activity compared to the control fish, which were not exposed to aluminum.
Step 5: Analyze the implications of the results. If the hypothesis is correct and there is a decrease in Na+/K+-ATPase activity in aluminum-exposed fish, this could explain physiological disturbances leading to mass die-offs, as the fish would struggle with disrupted ion balance and osmoregulation.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Aluminum Toxicity in Aquatic Organisms

Aluminum is a metal that can be toxic to aquatic life, particularly at elevated concentrations. In fish, exposure to high levels of aluminum can disrupt physiological processes, leading to stress and potentially fatal outcomes. This toxicity is often exacerbated in acidic waters, where aluminum becomes more soluble and bioavailable, impacting gill function and overall health.
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Na+/K+-ATPase Function

Na+/K+-ATPase is an essential enzyme found in the membranes of cells, including those in fish gills. It plays a critical role in maintaining the electrochemical gradient across the cell membrane by pumping sodium out and potassium into the cell. This process is vital for osmoregulation, nerve impulse transmission, and muscle contraction, making it a key indicator of cellular health and function in response to environmental stressors.
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Impact of Pollutants on Fish Physiology

Pollutants like aluminum can significantly affect fish physiology, particularly in their gills, which are crucial for respiration and ion regulation. Exposure to such pollutants can lead to altered enzyme activity, impaired gas exchange, and disrupted ion balance. The measurement of Na+/K+-ATPase activity in fish gills serves as a biomarker for assessing the physiological impact of pollutants, indicating how well the fish can cope with environmental stress.
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Textbook Question

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs such as the one pictured here. How does this occur? Which of the following is an osmoregulatory challenge that freshwater fishes need to overcome? a. diffusion of sodium ions out of the body b. diffusion of water out of the body c. active transport of sodium ions out of the body d. active transport of water out of the body

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Textbook Question

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs. In a laboratory, scientists exposed freshwater bony fish (Prochilodus lineatus) to water with a high level of aluminum and compared their blood osmolarity to that of fish exposed to water with a normal aluminum level (control). The results of the experiment are shown here (asterisks indicate P<0.05 between control and treated groups at a given time; BioSkills 3). Do the data support the hypothesis that aluminum interferes with osmoregulation in freshwater fishes? Explain.

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Textbook Question

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs. Why did the scientists do this experiment in a laboratory instead of simply collecting fish from a river with a high aluminum level and documenting their osmoregulatory ability?

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Textbook Question

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs. True or false: Water moves by osmosis across a fish's gills to an area with a higher sodium ion concentration because water molecules are attracted to the sodium ions.

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Textbook Question

Fish and other aquatic organisms are exposed to many types of water pollutants, including metals such as aluminum. Although a low level of aluminum is found in unpolluted water, many lakes and streams have an increased level because of mining, sewage treatment, and accidental spills of toxic materials. Aluminum pollution can result in mass fish die-offs. Draw a graph similar to the one here showing how the results would be different if the experiment had been performed on marine bony fish in seawater. (Assume that the osmolarity of seawater is 1100 mOsm and the set point osmolarity of marine bony fishes is 290 mOsm.)

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