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Leading & Lagging DNA Strands exam Flashcards

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Leading & Lagging DNA Strands exam
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  • Leading Strand


    The DNA strand that is synthesized continuously in the same direction as the replication fork.

  • Lagging Strand


    The DNA strand that is synthesized discontinuously in the opposite direction of the replication fork.

  • What is the direction of synthesis for the leading strand?


    In the same direction as the replication fork.

  • What is the direction of synthesis for the lagging strand?


    In the opposite direction of the replication fork.

  • RNA Primer


    A short segment of RNA required to initiate DNA synthesis.

  • How many RNA primers are required for the leading strand?


    One RNA primer.

  • How many RNA primers are required for the lagging strand?


    Multiple RNA primers.

  • Okazaki Fragments


    Short DNA segments synthesized on the lagging strand.

  • DNA Ligase


    The enzyme that joins Okazaki fragments together.

  • What enzyme joins Okazaki fragments?


    DNA ligase.

  • Replication Fork


    The area where the DNA double helix is unwound to allow replication.

  • What is the role of DNA polymerase in replication?


    It synthesizes new DNA strands in the 5' to 3' direction.

  • Origin of Replication


    The specific sequence of DNA where replication begins.

  • What is the origin of replication?


    The sequence of DNA where DNA replication begins.

  • Continuous Replication


    The process by which the leading strand is synthesized without interruption.

  • Discontinuous Replication


    The process by which the lagging strand is synthesized in short segments.

  • What is the difference between continuous and discontinuous replication?


    Continuous replication occurs on the leading strand, while discontinuous replication occurs on the lagging strand.

  • What are Okazaki fragments named after?


    A Japanese scientist named Okazaki.

  • What happens to RNA primers after DNA synthesis?


    They are removed and replaced with DNA nucleotides.

  • What is the function of the replication fork?


    To separate the DNA strands and allow replication to occur.

  • 5' to 3' Direction


    The direction in which DNA polymerase synthesizes new DNA strands.

  • Why does the lagging strand require multiple RNA primers?


    Because it is synthesized in short segments called Okazaki fragments.

  • What ensures genetic fidelity during DNA replication?


    The accurate synthesis of leading and lagging strands.

  • What is the role of DNA ligase in the lagging strand?


    To covalently anneal Okazaki fragments together.

  • What is the replication bubble?


    The region of DNA where replication is actively occurring, containing two replication forks.

  • What is the significance of the replication fork's direction?


    It determines the direction of synthesis for the leading and lagging strands.

  • What is the main challenge in synthesizing the lagging strand?


    It must be synthesized in short, discontinuous segments.

  • What enzyme is responsible for replacing RNA primers with DNA?


    DNA polymerase.