Introduction to Polymerase Chain Reaction definitions Flashcards

A molecule that carries genetic instructions for the development, functioning, growth, and reproduction of all known organisms and many viruses.

A segment of DNA that contains the instructions for building a specific protein or set of proteins, influencing traits and functions in an organism.

The complete set of an organism's DNA, including all of its genes and non-coding sequences, that contains the instructions for building and maintaining that organism.

A technique to rapidly amplify a specific DNA sequence in vitro, doubling the target DNA with each cycle, enabling extensive study without using living cells.

A technique to rapidly isolate and amplify a specific DNA sequence in a test tube, doubling the DNA amount with each cycle.

Amplify: To increase the number of copies of a specific DNA sequence through techniques like PCR, enabling detailed study and analysis.

A technique using living cells to create multiple copies of a specific DNA segment for study or manipulation.

A cylindrical glass or plastic container used in laboratories to hold, mix, or heat small quantities of liquid or solid chemicals, often used in PCR to amplify DNA sequences.

Non-coding DNA segments within a gene that are removed during RNA splicing.

A cell that provides the necessary environment and resources for a virus or foreign DNA to replicate and function.

A protein that cuts DNA at specific sequences, creating fragments with sticky or blunt ends, used in genetic engineering and molecular cloning.

The DNA strand used as a pattern for synthesizing a complementary strand during PCR, doubling with each cycle.

Determining the precise order of nucleotides in a DNA molecule, revealing the genetic information encoded within.

A series of steps in PCR where the DNA template is doubled, exponentially increasing the number of DNA copies with each repetition.

Overhanging single-stranded DNA sequences at the ends of double-stranded DNA fragments that can form hydrogen bonds with complementary sequences on other DNA fragments.