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Steps to DNA Cloning

18. Biotechnology / Steps to DNA Cloning / Problem 6

The expression of a cloned eukaryotic gene in a bacterial host cell is challenging due to the following factor(s):

A

Presence of introns in eukaryotes.

B

Differences in promoters and other DNA control sequences between prokaryotes and eukaryotes.

C

Post-transcriptional and post-translational modifications in eukaryotes.

D

All of the above.

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